Pump less steady-flow microfluidic chip for cell culture
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Marimuthu, Mohana | - |
dc.contributor.author | Kim, Sanghyo | - |
dc.date.available | 2020-02-28T23:44:58Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-06-15 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14472 | - |
dc.description.abstract | The current research engineered a pumpless energy-efficient microfluidic perfusion cell culture chip that works by modifying the basic gravity-driven siphon flow using an intravenous (IV) infusion set as a conventional, inexpensive, and sterile tool. The IV set was modified to control the constant hydrostatic head difference, thereby maintaining the steady flow rate medium perfusion. The micro-bioreactor chip demonstrated flexibility in controlling a wide range of flow rates from 0.1 to 10 ml/min, among which 1- and 5-ml/min flow rates were examined as suitable shear flows for long-term dermal fibroblast cell culture, paving the way for artificial skin development. (C) 2013 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | ANALYTICAL BIOCHEMISTRY | - |
dc.subject | PERFUSION CULTURE | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | SYSTEM | - |
dc.subject | PLATFORM | - |
dc.subject | ARRAYS | - |
dc.title | Pump less steady-flow microfluidic chip for cell culture | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000318744000011 | - |
dc.identifier.doi | 10.1016/j.ab.2013.02.007 | - |
dc.identifier.bibliographicCitation | ANALYTICAL BIOCHEMISTRY, v.437, no.2, pp.161 - 163 | - |
dc.identifier.scopusid | 2-s2.0-84876191666 | - |
dc.citation.endPage | 163 | - |
dc.citation.startPage | 161 | - |
dc.citation.title | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.volume | 437 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Marimuthu, Mohana | - |
dc.contributor.affiliatedAuthor | Kim, Sanghyo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Microfluidic | - |
dc.subject.keywordAuthor | Bioreactor | - |
dc.subject.keywordAuthor | Perfusion | - |
dc.subject.keywordAuthor | Flow rate | - |
dc.subject.keywordAuthor | Dermal fibroblasts | - |
dc.subject.keywordPlus | PERFUSION CULTURE | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | ARRAYS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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