N-ras Mutation Detection by Pyrosequencing in Adult Patients with Acute Myeloid Leukemia at a Single Institution
DC Field | Value | Language |
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dc.contributor.author | Jeong, Ji Hun | - |
dc.contributor.author | Park, Soon Ho | - |
dc.contributor.author | Park, Mi Jung | - |
dc.contributor.author | Kim, Moon Jin | - |
dc.contributor.author | Kim, Kyung Hee | - |
dc.contributor.author | Park, Pil Whan | - |
dc.contributor.author | Seo, Yiel Hea | - |
dc.contributor.author | Lee, Jae Hoon | - |
dc.contributor.author | Park, Jinny | - |
dc.contributor.author | Hong, Junshik | - |
dc.contributor.author | Ahn, Jeong Yeal | - |
dc.date.available | 2020-02-28T23:47:26Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-05 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14585 | - |
dc.description.abstract | Background: N-ras mutations are one of the most commonly detected abnormalities of myeloid origin. N-ras mutations result in a constitutively active N-ras protein that induces uncontrolled cell proliferation and inhibits apoptosis. We analyzed N-ras mutations in adult patients with AML at a particular institution and compared pyrosequencing analysis with a direct sequencing method for the detection of N-ras mutations. Methods: We analyzed 90 bone marrow samples from 83 AML patients. We detected N-ras mutations in codons 12, 13, and 61 using the pyrosequencing method and subsequently confirmed all data by direct sequencing. Using these methods, we screened the N-ras mutation quantitatively and determined the incidence and characteristic of N-ras mutation. Results: The incidence of N-ras mutation was 7.2% in adult AML patients. The patients with N-ras mutations showed significant higher hemoglobin levels (P=0.022) and an increased incidence of FLT3 mutations (P=0.003). We observed 3 cases with N-ras mutations in codon 12 (3.6%), 2 cases in codon 13 (2.4%), and 1 case in codon 61 (1.2%). All the mutations disappeared during chemotherapy. Conclusions: There is a low incidence (7.2%) of N-ras mutations in AML patients compared with other populations. Similar data is obtained by both pyrosequencing and direct sequencing. This study showed the correlation between the N-ras mutation and the therapeutic response. However, pyrosequencing provides quantitative data and is useful for monitoring therapeutic responses. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC LABORATORY MEDICINE | - |
dc.relation.isPartOf | ANNALS OF LABORATORY MEDICINE | - |
dc.subject | MYELOGENOUS-LEUKEMIA | - |
dc.subject | GENE MUTATION | - |
dc.subject | ONCOGENES | - |
dc.subject | YOUNGER | - |
dc.subject | FLT3 | - |
dc.title | N-ras Mutation Detection by Pyrosequencing in Adult Patients with Acute Myeloid Leukemia at a Single Institution | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000318888300001 | - |
dc.identifier.doi | 10.3343/alm.2013.33.3.159 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, v.33, no.3, pp.159 - 166 | - |
dc.identifier.kciid | ART001766994 | - |
dc.identifier.scopusid | 2-s2.0-84877992459 | - |
dc.citation.endPage | 166 | - |
dc.citation.startPage | 159 | - |
dc.citation.title | ANNALS OF LABORATORY MEDICINE | - |
dc.citation.volume | 33 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Jeong, Ji Hun | - |
dc.contributor.affiliatedAuthor | Park, Soon Ho | - |
dc.contributor.affiliatedAuthor | Park, Mi Jung | - |
dc.contributor.affiliatedAuthor | Kim, Moon Jin | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hee | - |
dc.contributor.affiliatedAuthor | Park, Pil Whan | - |
dc.contributor.affiliatedAuthor | Seo, Yiel Hea | - |
dc.contributor.affiliatedAuthor | Lee, Jae Hoon | - |
dc.contributor.affiliatedAuthor | Park, Jinny | - |
dc.contributor.affiliatedAuthor | Hong, Junshik | - |
dc.contributor.affiliatedAuthor | Ahn, Jeong Yeal | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | N-ras | - |
dc.subject.keywordAuthor | AML | - |
dc.subject.keywordAuthor | Pyrosequencing | - |
dc.subject.keywordAuthor | Bone marrow | - |
dc.subject.keywordPlus | MYELOGENOUS-LEUKEMIA | - |
dc.subject.keywordPlus | GENE MUTATION | - |
dc.subject.keywordPlus | ONCOGENES | - |
dc.subject.keywordPlus | YOUNGER | - |
dc.subject.keywordPlus | FLT3 | - |
dc.relation.journalResearchArea | Medical Laboratory Technology | - |
dc.relation.journalWebOfScienceCategory | Medical Laboratory Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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