Myeloid cell-specific serine palmitoyltransferase subunit 2 haploinsufficiency reduces murine atherosclerosis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chakraborty, Mahua | - |
dc.contributor.author | Lou, Caixia | - |
dc.contributor.author | Huan, Chongmin | - |
dc.contributor.author | Kuo, Ming-Shang | - |
dc.contributor.author | Park, Tae-Sik | - |
dc.contributor.author | Cao, Guoqing | - |
dc.contributor.author | Jiang, Xian-Cheng | - |
dc.date.available | 2020-02-29T00:42:03Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 0021-9738 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14649 | - |
dc.description.abstract | Serine palmitoyltransferase (SPT) is the first and rate-limiting enzyme of the de novo biosynthetic pathway of sphingomyelin (SM). Both SPT and SM have been implicated in the pathogenesis of atherosclerosis, the development of which is driven by macrophages; however, the role of SPT in macrophage-mediated atherogenesis is unknown. To address this issue, we have analyzed macrophage inflammatory responses and reverse cholesterol transport, 2 key mediators of atherogenesis, in SPT subunit 2-haploinsufficient (Sptlc2(+/-)) macrophages. We found that Sptlc2(+/-) macrophages have significantly lower SM levels in plasma membrane and lipid rafts. This reduction not only impaired inflammatory responses triggered by TLR4 and its downstream NF-kappa B and MAPK pathways, but also enhanced reverse cholesterol transport mediated by ABC transporters. LDL receptor-deficient (Ldlr(-/-)) mice transplanted with Sptlc2(+/-) bone marrow cells exhibited significantly fewer atherosclerotic lesions after high-fat and high-cholesterol diet feeding. Additionally, Ldlr(-/-) mice with myeloid cell-specific Sptlc2 haploinsufficiency exhibited significantly less atherosclerosis than controls. These findings suggest that SPT could be a novel therapeutic target in atherosclerosis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC CLINICAL INVESTIGATION INC | - |
dc.relation.isPartOf | JOURNAL OF CLINICAL INVESTIGATION | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | LOW-DENSITY-LIPOPROTEIN | - |
dc.subject | PALMITOYL-COA TRANSFERASE | - |
dc.subject | SCAVENGER RECEPTOR BI | - |
dc.subject | HAMSTER OVARY CELLS | - |
dc.subject | APOE-DEFICIENT MICE | - |
dc.subject | LIPID RAFTS | - |
dc.subject | PLASMA-MEMBRANE | - |
dc.subject | SPHINGOSINE-1-PHOSPHATE ANALOG | - |
dc.subject | ACCELERATES ATHEROSCLEROSIS | - |
dc.title | Myeloid cell-specific serine palmitoyltransferase subunit 2 haploinsufficiency reduces murine atherosclerosis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000317021800037 | - |
dc.identifier.doi | 10.1172/JCI60415 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CLINICAL INVESTIGATION, v.123, no.4, pp.1784 - 1797 | - |
dc.identifier.scopusid | 2-s2.0-84875843904 | - |
dc.citation.endPage | 1797 | - |
dc.citation.startPage | 1784 | - |
dc.citation.title | JOURNAL OF CLINICAL INVESTIGATION | - |
dc.citation.volume | 123 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Park, Tae-Sik | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | LOW-DENSITY-LIPOPROTEIN | - |
dc.subject.keywordPlus | PALMITOYL-COA TRANSFERASE | - |
dc.subject.keywordPlus | SCAVENGER RECEPTOR BI | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | APOE-DEFICIENT MICE | - |
dc.subject.keywordPlus | LIPID RAFTS | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE | - |
dc.subject.keywordPlus | SPHINGOSINE-1-PHOSPHATE ANALOG | - |
dc.subject.keywordPlus | ACCELERATES ATHEROSCLEROSIS | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.