Capacitive immunoaffinity biosensor based on vertically paired ring-electrodes
DC Field | Value | Language |
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dc.contributor.author | Lee, Ga-Yeon | - |
dc.contributor.author | Choi, Yong-Hwan | - |
dc.contributor.author | Chung, Ha-Wook | - |
dc.contributor.author | Ko, Hyuk | - |
dc.contributor.author | Cho, Sungbo | - |
dc.contributor.author | Pyun, Jae-Chul | - |
dc.date.available | 2020-02-29T00:44:13Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-02-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14747 | - |
dc.description.abstract | A capacitive biosensor was developed by using a vertically paired ring-electrode for the non-labeled immunoassay. The vertically paired ring-electrode was prepared by sequential deposition and etching processes. Two electrodes were layered on glass substrate by sputtering of gold layers with thicknesses of 50 nm and 100 nm, and a parylene-C film with a thickness of 550 nm was positioned between the electrode layers as a dielectric material by thermal deposition. The top layer was made by spin coating of SU-8. And then, the ring-electrodes were exposed at the wall of the layered structure by sequential etching processes. The fabricated electrodes were characterized by cyclic voltammetry of a well-known redox couple of 3,3',5,5'-tetramethylbenzidine. The non-labeled detection of antigen-antibody interaction was demonstrated by using anti-horseradish peroxidase (HRP) antibodies and C-reactive protein (CRP) as model analytes. When the model analytes were bound to the vertically paired ring-electrode, the impedance change was measured during the immunoassay steps, and the measured impedance was analyzed by using a model circuit of the ring-electrode, and the capacitance was estimated to be dependent on the adsorption of analytes between the ring-electrodes. (C) 2012 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.subject | IMPEDANCE SPECTROSCOPY | - |
dc.subject | DNA HYBRIDIZATION | - |
dc.subject | INTERFERON-GAMMA | - |
dc.subject | SPR BIOSENSOR | - |
dc.subject | IMMUNOSENSOR | - |
dc.subject | BINDING | - |
dc.subject | ARRAYS | - |
dc.subject | GOLD | - |
dc.subject | FILM | - |
dc.title | Capacitive immunoaffinity biosensor based on vertically paired ring-electrodes | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000312825800036 | - |
dc.identifier.doi | 10.1016/j.bios.2012.07.028 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.40, no.1, pp.227 - 232 | - |
dc.identifier.scopusid | 2-s2.0-84868644941 | - |
dc.citation.endPage | 232 | - |
dc.citation.startPage | 227 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 40 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Cho, Sungbo | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.subject.keywordAuthor | Capacitive | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordAuthor | Non-labeled detection | - |
dc.subject.keywordAuthor | Parylene-C | - |
dc.subject.keywordAuthor | Impedance | - |
dc.subject.keywordPlus | IMPEDANCE SPECTROSCOPY | - |
dc.subject.keywordPlus | DNA HYBRIDIZATION | - |
dc.subject.keywordPlus | INTERFERON-GAMMA | - |
dc.subject.keywordPlus | SPR BIOSENSOR | - |
dc.subject.keywordPlus | IMMUNOSENSOR | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | ARRAYS | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordPlus | FILM | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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