Noninvasive Identification of Viable Cell Populations in Docetaxel-Treated Breast Tumors Using Ferritin-Based Magnetic Resonance Imaging
DC Field | Value | Language |
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dc.contributor.author | Choi, YoonSeok | - |
dc.contributor.author | Kim, Hoe Suk | - |
dc.contributor.author | Cho, Kyoung-Won | - |
dc.contributor.author | Lee, Kyung-Min | - |
dc.contributor.author | Yi, Yoon Jung | - |
dc.contributor.author | Eun, Sung-Jong | - |
dc.contributor.author | Kim, Hyun Jin | - |
dc.contributor.author | Woo, Jisu | - |
dc.contributor.author | Choi, Seung Hong | - |
dc.contributor.author | Whangbo, Taeg-Keun | - |
dc.contributor.author | Choi, ChulSoo | - |
dc.contributor.author | Noh, Dong-Young | - |
dc.contributor.author | Moon, Woo Kyung | - |
dc.date.available | 2020-02-29T00:45:42Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-01-02 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14812 | - |
dc.description.abstract | Background: Cancer stem cells (CSCs) are highly tumorigenic and are responsible for tumor progression and chemoresistance. Noninvasive imaging methods for the visualization of CSC populations within tumors in vivo will have a considerable impact on the development of new CSC-targeting therapeutics. Methodology/Principal Findings: In this study, human breast cancer stem cells (BCSCs) transduced with dual reporter genes (human ferritin heavy chain [FTH] and enhanced green fluorescence protein [EGFP]) were transplanted into NOD/SCID mice to allow noninvasive tracking of BCSC-derived populations. No changes in the properties of the BCSCs were observed due to ferritin overexpression. Magnetic resonance imaging (MRI) revealed significantly different signal intensities (R-2* values) between BCSCs and FTH-BCSCs in vitro and in vivo. In addition, distinct populations of pixels with high R-2* values were detected in docetaxel-treated FTH-BCSC tumors compared with control tumors, even before the tumor sizes changed. Histological analysis revealed that areas showing high R-2* values in docetaxel-treated FTH-BCSC tumors by MRI contained EGFP+/FTH+ viable cell populations with high percentages of CD44+/CD24- cells. Conclusions/Significance: These findings suggest that ferritin-based MRI, which provides high spatial resolution and tissue contrast, can be used as a reliable method to identify viable cell populations derived from BCSCs after chemotherapy and may serve as a new tool to monitor the efficacy of CSC-targeting therapies in vivo. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.subject | CANCER STEM-CELLS | - |
dc.subject | IRON-OXIDE NANOPARTICLES | - |
dc.subject | MRI REPORTER | - |
dc.subject | CHEMOTHERAPY | - |
dc.subject | TRACKING | - |
dc.subject | QUANTIFICATION | - |
dc.subject | RESISTANCE | - |
dc.subject | RECEPTOR | - |
dc.title | Noninvasive Identification of Viable Cell Populations in Docetaxel-Treated Breast Tumors Using Ferritin-Based Magnetic Resonance Imaging | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000313320900046 | - |
dc.identifier.doi | 10.1371/journal.pone.0052931 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.8, no.1 | - |
dc.identifier.scopusid | 2-s2.0-84871791499 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 8 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Eun, Sung-Jong | - |
dc.contributor.affiliatedAuthor | Whangbo, Taeg-Keun | - |
dc.contributor.affiliatedAuthor | Choi, ChulSoo | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CANCER STEM-CELLS | - |
dc.subject.keywordPlus | IRON-OXIDE NANOPARTICLES | - |
dc.subject.keywordPlus | MRI REPORTER | - |
dc.subject.keywordPlus | CHEMOTHERAPY | - |
dc.subject.keywordPlus | TRACKING | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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