One-step solid phase-based on-chip sample preparation and integration with flow-through polymerase chain reaction
DC Field | Value | Language |
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dc.contributor.author | Trinh, K.T.L. | - |
dc.contributor.author | Tran, H.H. | - |
dc.contributor.author | Zhang, Y. | - |
dc.contributor.author | Wu, J. | - |
dc.contributor.author | Lee, N.Y. | - |
dc.date.available | 2020-02-29T00:47:02Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 0000-0000 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14880 | - |
dc.description.abstract | Sample preparation is one of the most labor-intensive and time-consuming processes yet indispensable for realizing a truly integrated micro total analyses systems (μTAS). In this study, we introduce Chelex resin as a solid support for one- step sample preparation. A plastic microdevice was fabricated using poly(methylmethacrylate) (PMMA), and Chelex resin was physically captured inside a microchannel. Using the fabricated microdevice, DNA was successfully extracted and purified from Escherichia coli and from human hairs. Furthermore, the sample preparation system was functionally integrated with flow-through polymerase chain reaction unit, and E. coli was successfully amplified in a seamless flow using the integrated microdevice. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Chemical and Biological Microsystems Society | - |
dc.relation.isPartOf | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 | - |
dc.title | One-step solid phase-based on-chip sample preparation and integration with flow-through polymerase chain reaction | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, v.1, pp.341 - 343 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84907361553 | - |
dc.citation.endPage | 343 | - |
dc.citation.startPage | 341 | - |
dc.citation.title | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 | - |
dc.citation.volume | 1 | - |
dc.contributor.affiliatedAuthor | Trinh, K.T.L. | - |
dc.contributor.affiliatedAuthor | Tran, H.H. | - |
dc.contributor.affiliatedAuthor | Zhang, Y. | - |
dc.contributor.affiliatedAuthor | Wu, J. | - |
dc.contributor.affiliatedAuthor | Lee, N.Y. | - |
dc.type.docType | Conference Paper | - |
dc.subject.keywordAuthor | Chelex resin | - |
dc.subject.keywordAuthor | Flow-through polymerase chain reaction | - |
dc.subject.keywordAuthor | Integrated plastic microdevice | - |
dc.subject.keywordAuthor | Sample preparation | - |
dc.subject.keywordPlus | Escherichia coli | - |
dc.subject.keywordPlus | Polymerase chain reaction | - |
dc.subject.keywordPlus | Resins | - |
dc.subject.keywordPlus | E. coli | - |
dc.subject.keywordPlus | Flowthrough | - |
dc.subject.keywordPlus | Human hair | - |
dc.subject.keywordPlus | Micro-device | - |
dc.subject.keywordPlus | On chips | - |
dc.subject.keywordPlus | Phase-based | - |
dc.subject.keywordPlus | Sample preparation | - |
dc.subject.keywordPlus | Solid supports | - |
dc.subject.keywordPlus | Polymers | - |
dc.description.journalRegisteredClass | scopus | - |
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