High-sensitivity chemiluminescence detection of cytokines using an antibody-immobilized CMOS image sensor
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hong, D.-G. | - |
dc.contributor.author | Joung, H.-A. | - |
dc.contributor.author | Kim, S.-H. | - |
dc.contributor.author | Kim, M.-G. | - |
dc.date.available | 2020-02-29T01:41:44Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0277-786X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14944 | - |
dc.description.abstract | In this study, we used a Complementary Metal Oxide Semiconductor (CMOS) image sensor with immobilizing antibodies on its surface to detect human cytokines, which are activators that mediate intercellular communication including expression and control of immune responses. The CMOS image sensor has many advantages over the Charge Couple Device, including lower power consumption, operation voltage, and cost. The photodiode, a unit pixel component in the CMOS image sensor, receives light from the detection area and generates digital image data. About a million pixels are embedded, and size of each pixel is 3 x 3 μm. The chemiluminescence reaction produces light from the chemical reaction of luminol and hydrogen peroxide. To detect cytokines, antibodies were immobilized on the surface of the CMOS image sensor, and a sandwich immunoassay using an HRP-labeled antibody was performed. An HRP-catalyzed chemiluminescence reaction was measured by each pixel of the CMOS image sensor. Pixels with stronger signals indicated higher cytokine concentrations; thus, we were able to measure human interleukin-5 (IL-5) at femtomolar concentrations. © 2013 SPIE. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.relation.isPartOf | Proceedings of SPIE - The International Society for Optical Engineering | - |
dc.subject | Chemiluminescence detection | - |
dc.subject | Chemiluminescence reactions | - |
dc.subject | Complementary metal oxide-semiconductor image sensor (CMOS) | - |
dc.subject | Digital image data | - |
dc.subject | Femtomolar concentration | - |
dc.subject | Intercellular communications | - |
dc.subject | Lower-power consumption | - |
dc.subject | Sandwich immunoassay | - |
dc.subject | Antibodies | - |
dc.subject | Chemical detection | - |
dc.subject | Chemiluminescence | - |
dc.subject | Digital cameras | - |
dc.subject | Pixels | - |
dc.title | High-sensitivity chemiluminescence detection of cytokines using an antibody-immobilized CMOS image sensor | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.doi | 10.1117/12.2018701 | - |
dc.identifier.bibliographicCitation | Proceedings of SPIE - The International Society for Optical Engineering, v.8879 | - |
dc.identifier.scopusid | 2-s2.0-84883759619 | - |
dc.citation.title | Proceedings of SPIE - The International Society for Optical Engineering | - |
dc.citation.volume | 8879 | - |
dc.contributor.affiliatedAuthor | Kim, S.-H. | - |
dc.type.docType | Conference Paper | - |
dc.subject.keywordPlus | Chemiluminescence detection | - |
dc.subject.keywordPlus | Chemiluminescence reactions | - |
dc.subject.keywordPlus | Complementary metal oxide-semiconductor image sensor (CMOS) | - |
dc.subject.keywordPlus | Digital image data | - |
dc.subject.keywordPlus | Femtomolar concentration | - |
dc.subject.keywordPlus | Intercellular communications | - |
dc.subject.keywordPlus | Lower-power consumption | - |
dc.subject.keywordPlus | Sandwich immunoassay | - |
dc.subject.keywordPlus | Antibodies | - |
dc.subject.keywordPlus | Chemical detection | - |
dc.subject.keywordPlus | Chemiluminescence | - |
dc.subject.keywordPlus | Digital cameras | - |
dc.subject.keywordPlus | Pixels | - |
dc.description.journalRegisteredClass | scopus | - |
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