女貞實이 LPS로 유발된 RAW 264.7 cell의 염증에 미치는 영향
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이용현 | - |
dc.contributor.author | 임은미 | - |
dc.date.available | 2020-02-29T01:45:55Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1229-4292 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/15184 | - |
dc.description.abstract | Purpose: This study was carried out to investigate the anti-inflammatory effectsof Ligustri Lucidi Fructus water extract (LF) in the lipopolysaccharide (LPS)-inducedmouse macrophages RAW 264.7 cell. Methods: Ligustri Lucidi Fructus was extracted with distilled water (2,000 ml)for 2 hours. In order to evaluate cytotoxicity of LF, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed. To investigateanti-inflammatory effects of LF, the concentration of nitric oxide (NO) was measuredwith NO assay, cytokine was measured by Bio-Plex cytokine assay, and intracellularcalcium (Ca) was measured with Fluo-4 Ca assay in RAW 264.7 cell. And when p-valueis below 0.05, it is judged to have the significant difference statistically (P<0.05). Results:1. LF showed no cytotoxicity. 2. LF inhibited significantly the production of NO at the concentration of 25,50 and 100 μg/ml. 3. LF inhibited significantly the production of interleukin (IL)-4, macrophageinflammatory protein (MIP)-1α, granulocyte colony stimulating factor (G-CSF)at the concentration of 25, 50, 100 and 200 μg/ml. 4. LF inhibited significantly the production of granulocyte macrophage-colony stimulatingfactor (GM-CSF), vascular endothelial growth factor (VEGF) at the concentrationof 50, 100 and 200 μg/ml, the interferon (IFN)-γ at 25, 50 and 100 μg/ml respectively. 5. LF inhibited significantly the production of IL-1β at the concentration of 50and 200 μg/ml, the IL-5 at 25 and 100 μg/ml, the IL-12p70, MIP-1β at 50 and100 μg/ml, the regulated on activation, normal T cell expressed and secrete d(RANTES) at 100 and 200 μg/ml respectively. 6. LF inhibited significantly the production of IL-10, interferon gamma-inducedprotein (IP)-10 at the concentration of 200 μg/ml. 7. LF inhibited significantly the production of intracellular Ca at the concentrationof 25, 50, 100 and 200 μg/ml. Conclusions: These results suggest that LF has anti-inflammatory effect andimmuno-modulating activity. | - |
dc.language | 한국어 | - |
dc.language.iso | ko | - |
dc.publisher | 대한한방부인과학회 | - |
dc.relation.isPartOf | 대한한방부인과학회지 | - |
dc.title | 女貞實이 LPS로 유발된 RAW 264.7 cell의 염증에 미치는 영향 | - |
dc.title.alternative | Anti-Inflammatory Effect of Ligustri Lucidi Fructus Water Extract in RAW 264.7 Cells Induced by LPS | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | 대한한방부인과학회지, v.26, no.4, pp.66 - 81 | - |
dc.identifier.kciid | ART001820523 | - |
dc.citation.endPage | 81 | - |
dc.citation.startPage | 66 | - |
dc.citation.title | 대한한방부인과학회지 | - |
dc.citation.volume | 26 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | 임은미 | - |
dc.subject.keywordAuthor | Ligustri Lucidi Fructus | - |
dc.subject.keywordAuthor | NO | - |
dc.subject.keywordAuthor | Cytokine | - |
dc.subject.keywordAuthor | Ca | - |
dc.subject.keywordAuthor | Anti-inflammation | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.