女貞實이 LPS로 유발된 RAW 264.7 cell의 염증에 미치는 영향

Abstract

Purpose: This study was carried out to investigate the anti-inflammatory effectsof Ligustri Lucidi Fructus water extract (LF) in the lipopolysaccharide (LPS)-inducedmouse macrophages RAW 264.7 cell. Methods: Ligustri Lucidi Fructus was extracted with distilled water (2,000 ml)for 2 hours. In order to evaluate cytotoxicity of LF, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed. To investigateanti-inflammatory effects of LF, the concentration of nitric oxide (NO) was measuredwith NO assay, cytokine was measured by Bio-Plex cytokine assay, and intracellularcalcium (Ca) was measured with Fluo-4 Ca assay in RAW 264.7 cell. And when p-valueis below 0.05, it is judged to have the significant difference statistically (P<0.05). Results:1. LF showed no cytotoxicity. 2. LF inhibited significantly the production of NO at the concentration of 25,50 and 100 μg/ml. 3. LF inhibited significantly the production of interleukin (IL)-4, macrophageinflammatory protein (MIP)-1α, granulocyte colony stimulating factor (G-CSF)at the concentration of 25, 50, 100 and 200 μg/ml. 4. LF inhibited significantly the production of granulocyte macrophage-colony stimulatingfactor (GM-CSF), vascular endothelial growth factor (VEGF) at the concentrationof 50, 100 and 200 μg/ml, the interferon (IFN)-γ at 25, 50 and 100 μg/ml respectively. 5. LF inhibited significantly the production of IL-1β at the concentration of 50and 200 μg/ml, the IL-5 at 25 and 100 μg/ml, the IL-12p70, MIP-1β at 50 and100 μg/ml, the regulated on activation, normal T cell expressed and secrete d(RANTES) at 100 and 200 μg/ml respectively. 6. LF inhibited significantly the production of IL-10, interferon gamma-inducedprotein (IP)-10 at the concentration of 200 μg/ml. 7. LF inhibited significantly the production of intracellular Ca at the concentrationof 25, 50, 100 and 200 μg/ml. Conclusions: These results suggest that LF has anti-inflammatory effect andimmuno-modulating activity.