熟地養筋湯 추출물이 흰쥐 좌골신경 손상 후에 기능회복과 염증 매개 인자 발현에 미치는 영향

Abstract

Objectives :Peripheral nerve injuries are commonly encountered clinical problems and often result in severe functional deficits. Sukjiyanggeun-Tang(shúdˋlyǎngj l̄n-tāng ), in oriental medicine, has been used to treat various musculoskeletal disorders. Methods :In the present study, the effects of aqueous extract of Sukjiyanggeun-Tang(shúdˋlyǎngj l̄n-tāng ) on functional recovery, severity of pain, and expressions of neurofilament, cycloxygenease-2(COX-2), inducible nitric oxide synthase(iNOS), and tumor necrosis factor-α(TNF-α) following sciatic crushed nerve injury in rats were investigated. For this study, walking tract analysis, plantar test, western blot analysis for COX-2 iNOS, and TNF-α, and Immunofluorescence test for neurofilament were performed. Results :In the present results, sciatic functional index(SFI) in walking tract analysis was significantly decreased following sciatic crushed nerve injury, and pain severity in plantar test was significantly increased. COX-2, iNOS and TNF-α expressions were increased whereas neurofilament expression was decreased by sciatic crushed nerve injury, In contrast, treatment with Sukjiyanggeun-Tang(shúdˋlyǎngj l̄n-tāng ) improved SFI in walking tract analysis and suppressed the pain severity in sciatic crushed nerve injury. Sukjiyanggeun-Tang(shúdˋlyǎngj l̄n-tāng ) treatment also suppressed COX-2, iNOS, and TNF-α expressions and enhanced the neurofilament expression in sciatic crushed nerve injury. Conclusions :In the present study, we have shown that Sukjiyanggeun-Tang(shúdˋlyǎngj l̄n-tāng ) is the effective therapeutic modality to ameliorate the symptoms of sciatic crushed nerve injury.