Effects of Ginsenoside-Rg1 on Post-thawed Miniature Pig Sperm Motility, Mitochondria Activity, and Membrane Integrity
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황유진 | - |
dc.contributor.author | 김대영 | - |
dc.date.available | 2020-02-29T03:43:34Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 2671-4639 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/15649 | - |
dc.description.abstract | In this study, we used flow a cytometric assay to evaluate plasma membrane integrity and mitochondrial activity in post-thawed sperm that was supplemented with ginsenoside-Rg1. Varying concentrations of ginsenoside-Rg1 (0, 25, 50 and 100 μM/ml) were used in the extender during cryopreservation to protect the DNA of thawed sperm, thereby increasing the viability and motility rate as evaluated using a computer-assisted sperm analysis (CASA) method. The results derived from CASA were used to compare the fresh, control, and ginsenoside-Rg1 groups. Sperm motility and the number of progressively motile sperm were significantly (p<0.05) higher in the 50 μM/ml ginsenoside-Rg1 group (61.0±4.65%) than in the control (46.6±7.02%), 25 μM/ml (46.2±4.76%), and 100 μM/ml ginsenoside-Rg1 (52.0± 1.90%) groups. However, the velocity distribution of post-thawed sperm did not differ significantly. Membrane integrity and MMP staining as revealed using flow cytometry were significantly (p<0.05) higher (91.6±0.82%) in the 50 μM/ml ginsenoside-Rg1 group than in the other groups. Here, we report that ginsenoside-Rg1 affects the motility and viability of boar spermatozoa. Moreover, ginsenoside- Rg1 can be used as a protective additive for the suppression of intracellular mitochondrial oxidative stress caused by cryopreservation | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 사단법인 한국동물생명공학회 | - |
dc.relation.isPartOf | 한국동물생명공학회지 | - |
dc.title | Effects of Ginsenoside-Rg1 on Post-thawed Miniature Pig Sperm Motility, Mitochondria Activity, and Membrane Integrity | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | 한국동물생명공학회지, v.28, no.1, pp.63 - 71 | - |
dc.identifier.kciid | ART001758162 | - |
dc.citation.endPage | 71 | - |
dc.citation.startPage | 63 | - |
dc.citation.title | 한국동물생명공학회지 | - |
dc.citation.volume | 28 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | 황유진 | - |
dc.contributor.affiliatedAuthor | 김대영 | - |
dc.subject.keywordAuthor | ginsenoside-Rg1 | - |
dc.subject.keywordAuthor | pig sperm | - |
dc.subject.keywordAuthor | CASA | - |
dc.subject.keywordAuthor | mitochondria activity | - |
dc.subject.keywordAuthor | membrane integrity | - |
dc.description.journalRegisteredClass | kci | - |
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