Caffeine-induced endothelial cell death and the inhibition of angiogenesis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hua Li | - |
dc.contributor.author | 진승유 | - |
dc.contributor.author | 손현준 | - |
dc.contributor.author | 서제훈 | - |
dc.contributor.author | 정구보 | - |
dc.date.available | 2020-02-29T03:43:38Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 2093-3665 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/15652 | - |
dc.description.abstract | Numerous studies have shown that adenosine or adenosine agonists can stimulate angiogenesis. However, the effect of caffeine (a known adenosine receptor antagonist) on angiogenesis has not been previously studied. Accordingly, this study was undertaken to examine the effect of caffeine on angiogenesis and to clarify the mechanism involved. Chick chorioallantoic membrane assays were used to investigate the effect of caffeine on angiogenesis and proliferation assays using human umbilical vein endothelial cells (HUVECs), were used to study its effects on specific aspects of angiogenesis. The expressions of caspase-3and Bcl-2 were examined by western blotting, immunofluorescence staining was used to identify HUVEC morphological changes, and fluorescence activated cell sorting (FACS) and DAPI staining were used to detect HUVEC apoptosis. Caffeine was found to inhibit blood vessel formation dose-dependently and to inhibit the proliferation of HUVECs time- and dosedependently. FACS analysis and DAPI staining showed that inhibitory effect of caffeine on HUVEC proliferation was the result of apoptosis and the up-regulation of thrombospondin-1 (TSP-1). Furthermore, TSP-1 levels were down-regulated by NECA but were unaffected by CGS21680, indicating that caffeine regulated TSP-1 expression via adenosine A2B receptor. In addition,caffeine up-regulated caspase-3 and down-regulated Bcl-2 at the protein level. These results suggest that the inhibitory effect of caffeine on angiogenesis is associated, at least in part, with its induction of endothelial cell apoptosis, probably mediated by a caspase-3 dependent mechanism. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 대한해부학회 | - |
dc.relation.isPartOf | Anatomy and Cell Biology | - |
dc.title | Caffeine-induced endothelial cell death and the inhibition of angiogenesis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | Anatomy and Cell Biology, v.46, no.1, pp.57 - 67 | - |
dc.identifier.kciid | ART001752126 | - |
dc.citation.endPage | 67 | - |
dc.citation.startPage | 57 | - |
dc.citation.title | Anatomy and Cell Biology | - |
dc.citation.volume | 46 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | 정구보 | - |
dc.subject.keywordAuthor | Angiogenesis | - |
dc.subject.keywordAuthor | Caffeine | - |
dc.subject.keywordAuthor | Human umbilical vein endothelial cells | - |
dc.subject.keywordAuthor | Thrombospondin 1 | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.