SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres
DC Field | Value | Language |
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dc.contributor.author | Ko, Juhui | - |
dc.contributor.author | Lee, Sangyeop | - |
dc.contributor.author | Lee, Eun Kyu | - |
dc.contributor.author | Chang, Soo-Ik | - |
dc.contributor.author | Chen, Lingxin | - |
dc.contributor.author | Yoon, Soo-Young | - |
dc.contributor.author | Choo, Jaebum | - |
dc.date.available | 2020-02-29T04:42:12Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 1463-9076 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/15856 | - |
dc.description.abstract | A novel SERS-based sandwich immunoassay using DNA aptamers, silica-encapsulated hollow gold nanospheres (SEHGNs) and a gold-patterned microarray was developed for sensitive detection of VEGF (vascular endothelial growth factor) angiogenesis protein markers. Here, a DNA aptamer conjugated to SEHGN was used as a highly reproducible SERS-encoding nanoprobe, and a hybrid microarray including hydrophilic gold wells and other hydrophobic areas was used as a SERS substrate. Target specific DNA aptamers that fold into a G-quadruplex structure were used as a target recognition unit instead of VEGF antibodies. The detection sensitivity was increased by 2 or 3 orders of magnitude over the conventional ELISA method. In particular, the dynamic concentration range was 3 or 4 orders of magnitude greater than that of conventional ELISA. The results demonstrate that this sensing strategy using DNA aptamers is a powerful platform for the design of novel immune-sensors with high performance. In particular, SERS-based detection using SEHGNs provides great promise for highly sensitive biomarker sensing with unprecedented advantages. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.relation.isPartOf | PHYSICAL CHEMISTRY CHEMICAL PHYSICS | - |
dc.title | SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000316467800012 | - |
dc.identifier.doi | 10.1039/c2cp43155f | - |
dc.identifier.bibliographicCitation | PHYSICAL CHEMISTRY CHEMICAL PHYSICS, v.15, no.15, pp.5379 - 5385 | - |
dc.identifier.scopusid | 2-s2.0-84875456719 | - |
dc.citation.endPage | 5385 | - |
dc.citation.startPage | 5379 | - |
dc.citation.title | PHYSICAL CHEMISTRY CHEMICAL PHYSICS | - |
dc.citation.volume | 15 | - |
dc.citation.number | 15 | - |
dc.contributor.affiliatedAuthor | Lee, Eun Kyu | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ENHANCED RAMAN-SCATTERING | - |
dc.subject.keywordPlus | PATTERNED MICROARRAY CHIP | - |
dc.subject.keywordPlus | CANCER MARKERS | - |
dc.subject.keywordPlus | NANOPROBES | - |
dc.subject.keywordPlus | SENSORS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | LUNG | - |
dc.subject.keywordPlus | BIOSENSORS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | GROWTH | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Physics, Atomic, Molecular & Chemical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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