MicroRNA-181c Inhibits Interleukin-6-mediated Beta Cell Apoptosis by Targeting TNF-alpha Expression
DC Field | Value | Language |
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dc.contributor.author | Oh, Yoon Sin | - |
dc.contributor.author | Bae, Gong Deuk | - |
dc.contributor.author | Park, Eun-Young | - |
dc.contributor.author | Jun, Hee-Sook | - |
dc.date.available | 2020-02-27T03:42:01Z | - |
dc.date.created | 2020-02-04 | - |
dc.date.issued | 2019-04-10 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1587 | - |
dc.description.abstract | We have previously reported that long-term treatment of beta cells with interleukin-6 (IL-6) is pro-apoptotic. However, little is known about the regulatory mechanisms that are involved. Therefore, we investigated pro-apoptotic changes in mRNA expression in beta cells in response to IL-6 treatment. We analyzed a microarray with RNA from INS-1 beta cells treated with IL-6, and found that TNF-alpha mRNA was significantly upregulated. Inhibition of TNF-alpha expression by neutralizing antibodies significantly decreased annexin V staining in cells compared with those treated with a control antibody. We identified three microRNAs that were differentially expressed in INS-1 cells incubated with IL-6. In particular, miR-181c was significantly downregulated in IL-6-treated cells compared with control cells and the decrease of miR-181c was attenuated by STAT-3 signaling inhibition. TNF-alpha mRNA was a direct target of miR-181c and upregulation of miR-181c by mimics, inhibited IL-6-induced increase in TNF-alpha mRNA expression. Consequently, reduction of TNF-alpha mRNA caused by miR-181c mimics enhanced cell viability in IL-6 treated INS-1 cells. These results demonstrated that miR-181c regulation of TNF-alpha expression plays a role in IL-6-induced beta cell apoptosis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | MOLECULES | - |
dc.subject | ISLET INFLAMMATION | - |
dc.subject | CYTOKINE | - |
dc.subject | TYPE-1 | - |
dc.subject | IL-6 | - |
dc.subject | STRESS | - |
dc.subject | REGULATORS | - |
dc.subject | MIR-181C | - |
dc.subject | PROTEIN | - |
dc.subject | DEATH | - |
dc.title | MicroRNA-181c Inhibits Interleukin-6-mediated Beta Cell Apoptosis by Targeting TNF-alpha Expression | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000464944500012 | - |
dc.identifier.doi | 10.3390/molecules24071410 | - |
dc.identifier.bibliographicCitation | MOLECULES, v.24, no.7 | - |
dc.identifier.scopusid | 2-s2.0-85064596919 | - |
dc.citation.title | MOLECULES | - |
dc.citation.volume | 24 | - |
dc.citation.number | 7 | - |
dc.contributor.affiliatedAuthor | Bae, Gong Deuk | - |
dc.contributor.affiliatedAuthor | Jun, Hee-Sook | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Interleukin-6 | - |
dc.subject.keywordAuthor | beta cell apoptosis | - |
dc.subject.keywordAuthor | microRNA-181c | - |
dc.subject.keywordPlus | ISLET INFLAMMATION | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | TYPE-1 | - |
dc.subject.keywordPlus | IL-6 | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | REGULATORS | - |
dc.subject.keywordPlus | MIR-181C | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | DEATH | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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