The transcription factor Sp1 is responsible for aging-dependent altered nucleocytoplasmic trafficking
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sung Y. | - |
dc.contributor.author | Kang, Hyun T. | - |
dc.contributor.author | Han, Jeong A. | - |
dc.contributor.author | Park, Sang C. | - |
dc.date.available | 2020-02-29T04:44:49Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-12 | - |
dc.identifier.issn | 1474-9718 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/15968 | - |
dc.description.abstract | Hyporesponsiveness to external signals, such as growth factors and apoptotic stimuli, is a cardinal feature of cellular senescence. We previously reported that an aging-dependent marked reduction in nucleocytoplasmic trafficking (NCT)-related genes could be responsible for this phenomenon. In searching for the mechanism, we identified the transcription factor, Sp1, as a common regulator of NCT genes, including various nucleoporins, importins, exportins, and Ran GTPase cycle-related genes. Sp1 knockdown led to a reduction of those genes in young human diploid fibroblast cells (HDF); Sp1 overexpression induced those genes in senescent cells. In addition, epidermal growth factor stimulationinduced p-ERK1/2 nuclear translocation and Elk-1 phosphorylation were severely impaired by Sp1 depletion in young HDFs; Sp1 overexpression restored the nuclear translocation of p-ERK1/2 in senescent HDFs. Furthermore, we observed that Sp1 protein levels were decreased in senescent cells, and H2O2 treatment decreased Sp1 levels in a proteasome-dependent manner. In addition, O-GlcNAcylation of Sp1 was decreased in senescent cells as well as in H2O2-treated cells. Taken together, these results suggest that Sp1 could be a key regulator in the control of NCT genes and that reactive oxygen species-mediated alteration in Sp1 stability may be responsible for the generalized repression of those genes, leading to formation of the senescence-dependent functional nuclear barrier, resulting in subsequent hyporesponsiveness to external signals. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.relation.isPartOf | AGING CELL | - |
dc.subject | CELLULAR SENESCENCE | - |
dc.subject | HUMAN FIBROBLASTS | - |
dc.subject | ACTIVATION | - |
dc.subject | CELLS | - |
dc.subject | RESISTANCE | - |
dc.subject | APOPTOSIS | - |
dc.subject | COMPLEX | - |
dc.subject | STRESS | - |
dc.subject | GENE | - |
dc.title | The transcription factor Sp1 is responsible for aging-dependent altered nucleocytoplasmic trafficking | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000311113500020 | - |
dc.identifier.doi | 10.1111/acel.12012 | - |
dc.identifier.bibliographicCitation | AGING CELL, v.11, no.6, pp.1102 - 1109 | - |
dc.identifier.scopusid | 2-s2.0-84869198829 | - |
dc.citation.endPage | 1109 | - |
dc.citation.startPage | 1102 | - |
dc.citation.title | AGING CELL | - |
dc.citation.volume | 11 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Kim, Sung Y. | - |
dc.contributor.affiliatedAuthor | Kang, Hyun T. | - |
dc.contributor.affiliatedAuthor | Park, Sang C. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | aging | - |
dc.subject.keywordAuthor | cellular senescence | - |
dc.subject.keywordAuthor | nuclear pore complex | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | Sp1 | - |
dc.subject.keywordPlus | CELLULAR SENESCENCE | - |
dc.subject.keywordPlus | HUMAN FIBROBLASTS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | GENE | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Geriatrics & Gerontology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Geriatrics & Gerontology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.