Glucose-responsive artificial promoter-mediated insulin gene transfer improves glucose control in diabetic mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Jaeseok | - |
dc.contributor.author | Kim, Eung-Hwi | - |
dc.contributor.author | Choi, Woohyuk | - |
dc.contributor.author | Jun, Hee-Sook | - |
dc.date.available | 2020-02-29T04:45:21Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-11-28 | - |
dc.identifier.issn | 1007-9327 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/15992 | - |
dc.description.abstract | AIM: To investigate the effect of insulin gene therapy using a glucose-responsive synthetic promoter in type 2 diabetic obese mice. METHODS: We employed a recently developed novel insulin gene therapy strategy using a synthetic promoter that regulates insulin gene expression in the liver in response to blood glucose level changes. We intravenously administered a recombinant adenovirus expressing furin-cleavable rat insulin under the control of the synthetic promoter (rAd-SP-rINSfur) into diabetic Lepr(db/db) mice. A recombinant adenovirus expressing beta-galactosidase under the cytomegalovirus promoter was used as a control (rAd-CMV-beta gal). Blood glucose levels and body weights were monitored for 50 d. Glucose and insulin tolerance tests were performed. Immunohistochemical staining was performed to investigate islet morphology and insulin content. RESULTS: Administration of rAd-SP-rINSfur lowered blood glucose levels and normoglycemia was maintained for 50 d, whereas the rAd-CMV-beta gal control virus-injected mice remained hyperglycemic. Glucose tolerance tests showed that rAd-SP-rINSfur-treated mice cleared exogenous glucose from the blood more efficiently than control virus-injected mice at 4 wk [area under the curve (AUC): 21 508.80 +/- 2248.18 vs 62 640.00 +/- 5014.28, P < 0.01] and at 6 wk (AUC: 29 956.60 +/- 1757.33 vs 60 016.60 +/- 3794.47, P < 0.01). In addition, insulin sensitivity was also significantly improved in mice treated with rAd-SP-rINSfur compared with rAd-CMV-beta gal-treated mice (AUC: 9150.17 +/- 1007.78 vs 11 994.20 +/- 474.40, P < 0.05). The islets from rAd-SP-rINSfur-injected mice appeared to be smaller and to contain a higher concentration of insulin than those from rAd-CMV-beta gal-injected mice. CONCLUSION: Based on these results, we suggest that insulin gene therapy might be one therapeutic option for remission of type 2 diabetes. (C) 2012 Baishideng. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BAISHIDENG PUBLISHING GROUP INC | - |
dc.relation.isPartOf | WORLD JOURNAL OF GASTROENTEROLOGY | - |
dc.subject | CARDIOVASCULAR-DISEASE | - |
dc.subject | THERAPY | - |
dc.subject | HYPERGLYCEMIA | - |
dc.subject | RESISTANCE | - |
dc.subject | PATHOPHYSIOLOGY | - |
dc.subject | ASSOCIATION | - |
dc.subject | SECRETION | - |
dc.subject | RISK | - |
dc.title | Glucose-responsive artificial promoter-mediated insulin gene transfer improves glucose control in diabetic mice | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000312042200008 | - |
dc.identifier.doi | 10.3748/wjg.v18.i44.6420 | - |
dc.identifier.bibliographicCitation | WORLD JOURNAL OF GASTROENTEROLOGY, v.18, no.44, pp.6420 - 6426 | - |
dc.identifier.scopusid | 2-s2.0-84873904540 | - |
dc.citation.endPage | 6426 | - |
dc.citation.startPage | 6420 | - |
dc.citation.title | WORLD JOURNAL OF GASTROENTEROLOGY | - |
dc.citation.volume | 18 | - |
dc.citation.number | 44 | - |
dc.contributor.affiliatedAuthor | Kim, Eung-Hwi | - |
dc.contributor.affiliatedAuthor | Choi, Woohyuk | - |
dc.contributor.affiliatedAuthor | Jun, Hee-Sook | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Insulin gene therapy | - |
dc.subject.keywordAuthor | Synthetic promoter | - |
dc.subject.keywordAuthor | Glucose-responsive element | - |
dc.subject.keywordAuthor | Liver-specific promoter | - |
dc.subject.keywordAuthor | Type 2 diabetes | - |
dc.subject.keywordPlus | CARDIOVASCULAR-DISEASE | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | HYPERGLYCEMIA | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | PATHOPHYSIOLOGY | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | RISK | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.