Large-scale production of soluble recombinant amyloid-beta peptide 1-42 using cold-inducible expression system
DC Field | Value | Language |
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dc.contributor.author | Kim, Eun-Kyung | - |
dc.contributor.author | Moon, Jeong Chan | - |
dc.contributor.author | Lee, Jeong Mi | - |
dc.contributor.author | Jeong, Min Seop | - |
dc.contributor.author | Oh, Choongseob | - |
dc.contributor.author | Ahn, Sung-Min | - |
dc.contributor.author | Yoo, Yung Joon | - |
dc.contributor.author | Jang, Ho Hee | - |
dc.date.available | 2020-02-29T04:46:45Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-11 | - |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16046 | - |
dc.description.abstract | Amyloid-beta peptide 1-42 (A beta(1-42)), the predominant form in senile plaques, plays important roles in the pathogenesis of Alzheimer's disease. Because A beta(1-42) has aggregation-prone nature, it has been difficult to produce in a soluble state in bacterial expression systems. In this study, we modified our expression system to increase the soluble fraction of A beta(1-42) in Escherichia coli (E. coli) cells. The expression level and solubility of recombinant A beta(1-42) induced at the low temperature (16 degrees C) is highly increased compared to that induced at 37 degrees C. To optimize expression temperature, the coding region of A beta(1-42) was constructed in a pCold vector, pCold-TF, which has a hexahistidine-tagged trigger factor (TF). Recombinant A beta(1-42) was expressed primarily as a soluble protein using pCold vector system and purified with a nickel-chelating resin. When the toxic effect of recombinant A beta(1-42) examined on human neuroblastoma SH-SY5Y cells, the purified A beta(1-42) induced cell toxicity on SH-SY5Y cells. In conclusion, the system developed in this study will provide a useful method for the production of aggregation prone-peptide such as A beta(1-42). (C) 2012 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.subject | PROTEIN | - |
dc.subject | AGGREGATION | - |
dc.subject | PURIFICATION | - |
dc.subject | CONVERSION | - |
dc.subject | A-BETA-42 | - |
dc.title | Large-scale production of soluble recombinant amyloid-beta peptide 1-42 using cold-inducible expression system | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000310654600008 | - |
dc.identifier.doi | 10.1016/j.pep.2012.08.021 | - |
dc.identifier.bibliographicCitation | PROTEIN EXPRESSION AND PURIFICATION, v.86, no.1, pp.53 - 57 | - |
dc.identifier.scopusid | 2-s2.0-84867892987 | - |
dc.citation.endPage | 57 | - |
dc.citation.startPage | 53 | - |
dc.citation.title | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.citation.volume | 86 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Kim, Eun-Kyung | - |
dc.contributor.affiliatedAuthor | Moon, Jeong Chan | - |
dc.contributor.affiliatedAuthor | Lee, Jeong Mi | - |
dc.contributor.affiliatedAuthor | Jeong, Min Seop | - |
dc.contributor.affiliatedAuthor | Oh, Choongseob | - |
dc.contributor.affiliatedAuthor | Ahn, Sung-Min | - |
dc.contributor.affiliatedAuthor | Jang, Ho Hee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Aggregation-prone | - |
dc.subject.keywordAuthor | Alzheimer&apos | - |
dc.subject.keywordAuthor | s disease | - |
dc.subject.keywordAuthor | Amyloid-beta peptide 1-42 | - |
dc.subject.keywordAuthor | Cell viability | - |
dc.subject.keywordAuthor | Cold induction | - |
dc.subject.keywordAuthor | Soluble expression | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | A-BETA-42 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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