SERS-based multiple biomarker detection using a gold-patterned microarray chip
DC Field | Value | Language |
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dc.contributor.author | Kim, Insup | - |
dc.contributor.author | Junejo, Inam-ur-Rehman | - |
dc.contributor.author | Lee, Moonkwon | - |
dc.contributor.author | Lee, Sangyeop | - |
dc.contributor.author | Lee, Eun Kyu | - |
dc.contributor.author | Chang, Soo-Ik | - |
dc.contributor.author | Choo, Jaebum | - |
dc.date.available | 2020-02-29T05:43:14Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-09-12 | - |
dc.identifier.issn | 0022-2860 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16166 | - |
dc.description.abstract | We report a highly sensitive surface-enhanced Raman scattering (SERS)-based immunoassay platform for the multiplex detection of biomarkers. For this purpose, a gold-patterned microarray chip has been fabricated and used as a SERS detection template. Here, a typical sandwich immunocomplex protocol was adopted. Monoclonal antibodies were immobilized on gold patterned substrates, and then antigen solutions and polyclonal antibody-conjugated hollow gold nanospheres (HGNs) were sequentially added for the formation of sandwich immunocomplexes. Antigen biomarkers can be quantitatively assayed by monitoring the intensity change of a characteristic SERS peak of a reporter molecule adsorbed on the surfaces of HGNs. Under optimized assay conditions, the limits of detections (LODs) were determined to be 10 fg/mL for human IgG and 10-100 fg/mL for rabbit IgG. In addition, the SERS-based immunoassay technique can be applied in a wider dynamic concentration range with a good sensitivity compared to ELISA. The proposed method fulfills the current needs of high sensitivity and selectivity which are essential for the clinical diagnosis of a disease. (c) 2012 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.relation.isPartOf | JOURNAL OF MOLECULAR STRUCTURE | - |
dc.subject | ENHANCED RAMAN-SCATTERING | - |
dc.subject | CANCER MARKERS | - |
dc.subject | CARCINOEMBRYONIC ANTIGEN | - |
dc.subject | IMMUNOASSAY | - |
dc.subject | NANOPARTICLES | - |
dc.subject | TECHNOLOGY | - |
dc.subject | NANOPROBES | - |
dc.title | SERS-based multiple biomarker detection using a gold-patterned microarray chip | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000308971900031 | - |
dc.identifier.doi | 10.1016/j.molstruc.2012.04.031 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MOLECULAR STRUCTURE, v.1023, pp.197 - 203 | - |
dc.identifier.scopusid | 2-s2.0-84864627552 | - |
dc.citation.endPage | 203 | - |
dc.citation.startPage | 197 | - |
dc.citation.title | JOURNAL OF MOLECULAR STRUCTURE | - |
dc.citation.volume | 1023 | - |
dc.contributor.affiliatedAuthor | Lee, Eun Kyu | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Microarray chip | - |
dc.subject.keywordAuthor | Hollow gold nanospheres | - |
dc.subject.keywordAuthor | Surface-enhanced Raman scattering | - |
dc.subject.keywordAuthor | Multiplex assay | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordAuthor | ELISA | - |
dc.subject.keywordPlus | ENHANCED RAMAN-SCATTERING | - |
dc.subject.keywordPlus | CANCER MARKERS | - |
dc.subject.keywordPlus | CARCINOEMBRYONIC ANTIGEN | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | TECHNOLOGY | - |
dc.subject.keywordPlus | NANOPROBES | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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