TGF-beta sensitivity is determined by N-linked glycosylation of the type II TGF-beta receptor
DC Field | Value | Language |
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dc.contributor.author | Kim, Young-Woong | - |
dc.contributor.author | Park, Jinah | - |
dc.contributor.author | Lee, Hyun-Ju | - |
dc.contributor.author | Lee, So-Young | - |
dc.contributor.author | Kim, Seong-Jin | - |
dc.date.available | 2020-02-29T05:44:49Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-08-01 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16233 | - |
dc.description.abstract | N-linked 21ycosylation is a critical determinant of protein structure and function, regulating processes such as protein folding, stability and localization, ligand receptor binding and intracellular signalling. T beta RII [type II TGF-beta (transforming growth factor beta) receptor] plays a crucial role in the TGF-beta signalling pathway. Although N-linked glycosylation of T beta R11 was first demonstrated over a decade ago, it was unclear how this modification influenced T beta RII biology. In the present study, we show that inhibiting the N-linked glycosylation process successfully hinders binding of TGF-beta 1 to T beta RII and subsequently renders cells resistant to TGF-beta signalling. The lung cancer eel:. line A549, the gastric carcinoma cell line MKN1 and the immortal cell line HEK (human embryonic kidney)-293 exhibit reduced TGF-beta signalling when either treated with two inhibitors, including tunicamycin (a potent N-linked glycosylation inhibitor) and kifunensine [an inhibitor of ER (endoplasmic reticulum) and Golgi mannosidase I family members], or introduced with a non-glycosylated mutant version of T beta RII. We demonstrate that defective N-linked glycosylation prevents T beta RII proteins from being transported to the cell surface. Moreover, we clearly show that not only the complex type, but also a high-mannose type, of T beta RII can be localized on the cell surface. Collectively, these findings demonstrate that N-linked glycosylation is essentially required for the successful cell surface transportation of T beta RII, suggesting a novel mechanism by which the TGF-beta sensitivity can be regulated by N-linked glycosylation levels of T beta RII. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PORTLAND PRESS LTD | - |
dc.relation.isPartOf | BIOCHEMICAL JOURNAL | - |
dc.subject | CELL-SURFACE TRANSPORT | - |
dc.subject | TUMOR-SUPPRESSOR | - |
dc.subject | MEMBRANE | - |
dc.subject | OLIGOSACCHARIDES | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | MECHANISMS | - |
dc.subject | EXPRESSION | - |
dc.subject | STABILITY | - |
dc.subject | PHENOTYPE | - |
dc.subject | PROTEINS | - |
dc.title | TGF-beta sensitivity is determined by N-linked glycosylation of the type II TGF-beta receptor | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000307036600012 | - |
dc.identifier.doi | 10.1042/BJ20111923 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL JOURNAL, v.445, pp.403 - 411 | - |
dc.identifier.scopusid | 2-s2.0-84864345961 | - |
dc.citation.endPage | 411 | - |
dc.citation.startPage | 403 | - |
dc.citation.title | BIOCHEMICAL JOURNAL | - |
dc.citation.volume | 445 | - |
dc.contributor.affiliatedAuthor | Kim, Young-Woong | - |
dc.contributor.affiliatedAuthor | Park, Jinah | - |
dc.contributor.affiliatedAuthor | Lee, Hyun-Ju | - |
dc.contributor.affiliatedAuthor | Kim, Seong-Jin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | cell surface transport | - |
dc.subject.keywordAuthor | N-linked glycosylation | - |
dc.subject.keywordAuthor | transforming growth factor beta sensitivity (TGF-beta sensitivity) | - |
dc.subject.keywordAuthor | transforming growth factor beta signalling (TGF-beta signalling) | - |
dc.subject.keywordAuthor | type II transforming growth factor beta receptor (T beta RII) | - |
dc.subject.keywordPlus | CELL-SURFACE TRANSPORT | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | PHENOTYPE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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