Prognostic Significance of Glutathione Peroxidase 1 (GPX1) Down-regulation and Correlation with Aberrant Promoter Methylation in Human Gastric Cancer
DC Field | Value | Language |
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dc.contributor.author | Min, Sun Young | - |
dc.contributor.author | Kim, Hee Sung | - |
dc.contributor.author | Jung, Eun Jung | - |
dc.contributor.author | Jung, Eun Ji | - |
dc.contributor.author | Do Jee, Chang | - |
dc.contributor.author | Kim, Woo Ho | - |
dc.date.available | 2020-02-29T05:45:03Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-08 | - |
dc.identifier.issn | 0250-7005 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16250 | - |
dc.description.abstract | Background: This study aimed at examining the association of gene silencing and promoter methylation of glutathione peroxidase 1 (GPX1) and glutathione peroxidase 3 (GPX3) in gastric cancer cells and determined the clinical significance of GPX1 and GPX3 expression loss in gastric cancer tissue. Materials and Methods: Analysis of mRNA expression was carried out by reverse transcription-polymerase chain reaction (RT-PCR). Methylation of the GPX1 promoter region was analyzed by bisulfite sequencing, and that of the GPX3 promoter region was analyzed by methylation-specific PCR (MSP). Tissue microarray-based immunohistochemistry of GPX1 and GPX3 in 1,163 resected gastric cancer specimens was performed to assess the associations with clinicopathological parameters. Results: Reduced GPX1 and GPX3 mRNA expression was associated with promoter methylation in gastric cancer cell lines. A correlation between DNA promoter methylation and loss of GPX1 expression was noted in 16 gastric cancer tissue samples (p=0.005). Loss of GPX1 and GPX3 proteins was found in 24.4% and 30.8% of gastric cancer tissues. Loss of GPX1 expression was significantly associated with advanced gastric cancer (p=0.039) and lymphatic invasion (p=0.010); loss of GPX3 expression was associated with advanced gastric cancer (p<0.001) and lymph node metastasis (p<0.001). Kaplan-Meier analysis showed that low expression of GPX1 was associated with poor cancer-specific survival (p=0.010). Conclusion: Data from this study implicate aberrant hypermethylation of promoter regions of GPX1 and GPX3 as a mechanism for down-regulation of GPX1 and GPX3 mRNA expression in gastric cancer cells. Loss of GPX1 expression was associated with aggressiveness and poor survival in patients with gastric cancer. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | INT INST ANTICANCER RESEARCH | - |
dc.relation.isPartOf | ANTICANCER RESEARCH | - |
dc.subject | HELICOBACTER-PYLORI-INFECTION | - |
dc.subject | CPG ISLAND HYPERMETHYLATION | - |
dc.subject | PROSTATE-CANCER | - |
dc.subject | EXPRESSION | - |
dc.subject | GLUTATHIONE-PEROXIDASE-3 | - |
dc.subject | SELENOPROTEINS | - |
dc.subject | CARCINOGENESIS | - |
dc.subject | TUMORIGENESIS | - |
dc.subject | ASSOCIATION | - |
dc.subject | SELENIUM | - |
dc.title | Prognostic Significance of Glutathione Peroxidase 1 (GPX1) Down-regulation and Correlation with Aberrant Promoter Methylation in Human Gastric Cancer | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000306892700021 | - |
dc.identifier.bibliographicCitation | ANTICANCER RESEARCH, v.32, no.8, pp.3169 - 3175 | - |
dc.identifier.scopusid | 2-s2.0-84865680715 | - |
dc.citation.endPage | 3175 | - |
dc.citation.startPage | 3169 | - |
dc.citation.title | ANTICANCER RESEARCH | - |
dc.citation.volume | 32 | - |
dc.citation.number | 8 | - |
dc.contributor.affiliatedAuthor | Kim, Hee Sung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Glutathione peroxidase 1 | - |
dc.subject.keywordAuthor | glutathione peroxidase 3 | - |
dc.subject.keywordAuthor | stomach cancer | - |
dc.subject.keywordAuthor | methylation | - |
dc.subject.keywordPlus | HELICOBACTER-PYLORI-INFECTION | - |
dc.subject.keywordPlus | CPG ISLAND HYPERMETHYLATION | - |
dc.subject.keywordPlus | PROSTATE-CANCER | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GLUTATHIONE-PEROXIDASE-3 | - |
dc.subject.keywordPlus | SELENOPROTEINS | - |
dc.subject.keywordPlus | CARCINOGENESIS | - |
dc.subject.keywordPlus | TUMORIGENESIS | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | SELENIUM | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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