Facile backbone structure determination of human membrane proteins by NMR spectroscopy
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Klammt, Christian | - |
dc.contributor.author | Maslennikov, Innokentiy | - |
dc.contributor.author | Bayrhuber, Monika | - |
dc.contributor.author | Eichmann, Cedric | - |
dc.contributor.author | Vajpai, Navratna | - |
dc.contributor.author | Chiu, Ellis Jeremy Chua | - |
dc.contributor.author | Blain, Katherine Y. | - |
dc.contributor.author | Esquivies, Luis | - |
dc.contributor.author | Kwon, June Hyun Jung | - |
dc.contributor.author | Balana, Bartosz | - |
dc.contributor.author | Pieper, Ursula | - |
dc.contributor.author | Sali, Andrej | - |
dc.contributor.author | Slesinger, Paul A. | - |
dc.contributor.author | Kwiatkowski, Witek | - |
dc.contributor.author | Riek, Roland | - |
dc.contributor.author | Choe, Senyon | - |
dc.date.available | 2020-02-29T05:45:24Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-08 | - |
dc.identifier.issn | 1548-7091 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16259 | - |
dc.description.abstract | Although nearly half of today's major pharmaceutical drugs target human integral membrane proteins (hIMPs), only 30 hIMP structures are currently available in the Protein Data Bank, largely owing to inefficiencies in protein production. Here we describe a strategy for the rapid structure determination of hIMPs, using solution NMR spectroscopy with systematically labeled proteins produced via cell-free expression. We report new backbone structures of six hIMPs, solved in only 18 months from 15 initial targets. Application of our protocols to an additional 135 hIMPs with molecular weight <30 kDa yielded 38 hIMPs suitable for structural characterization by solution NMR spectroscopy without additional optimization. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.relation.isPartOf | NATURE METHODS | - |
dc.subject | CELL-FREE EXPRESSION | - |
dc.subject | PARAMAGNETIC RELAXATION ENHANCEMENT | - |
dc.subject | TRIPLE-RESONANCE EXPERIMENTS | - |
dc.subject | NUCLEAR-MAGNETIC-RESONANCE | - |
dc.subject | FUNCTIONAL-ANALYSIS | - |
dc.subject | COUPLED RECEPTORS | - |
dc.subject | PREPARATIVE-SCALE | - |
dc.subject | HUMAN ENDOTHELIN | - |
dc.subject | DETERGENTS | - |
dc.subject | ASSIGNMENT | - |
dc.title | Facile backbone structure determination of human membrane proteins by NMR spectroscopy | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000307015700028 | - |
dc.identifier.doi | 10.1038/NMETH.2033 | - |
dc.identifier.bibliographicCitation | NATURE METHODS, v.9, no.8, pp.834 - + | - |
dc.identifier.scopusid | 2-s2.0-84864426351 | - |
dc.citation.endPage | + | - |
dc.citation.startPage | 834 | - |
dc.citation.title | NATURE METHODS | - |
dc.citation.volume | 9 | - |
dc.citation.number | 8 | - |
dc.contributor.affiliatedAuthor | Klammt, Christian | - |
dc.contributor.affiliatedAuthor | Maslennikov, Innokentiy | - |
dc.contributor.affiliatedAuthor | Chiu, Ellis Jeremy Chua | - |
dc.contributor.affiliatedAuthor | Kwon, June Hyun Jung | - |
dc.contributor.affiliatedAuthor | Choe, Senyon | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CELL-FREE EXPRESSION | - |
dc.subject.keywordPlus | PARAMAGNETIC RELAXATION ENHANCEMENT | - |
dc.subject.keywordPlus | TRIPLE-RESONANCE EXPERIMENTS | - |
dc.subject.keywordPlus | NUCLEAR-MAGNETIC-RESONANCE | - |
dc.subject.keywordPlus | FUNCTIONAL-ANALYSIS | - |
dc.subject.keywordPlus | COUPLED RECEPTORS | - |
dc.subject.keywordPlus | PREPARATIVE-SCALE | - |
dc.subject.keywordPlus | HUMAN ENDOTHELIN | - |
dc.subject.keywordPlus | DETERGENTS | - |
dc.subject.keywordPlus | ASSIGNMENT | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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