The association between prion proteins and A beta(1-42) oligomers in cytotoxicity and apoptosis
DC Field | Value | Language |
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dc.contributor.author | Hyeon, Jae Wook | - |
dc.contributor.author | Kim, Su Yeon | - |
dc.contributor.author | Park, Jun Sun | - |
dc.contributor.author | Choi, Bo Yeong | - |
dc.contributor.author | Lee, Sol Moe | - |
dc.contributor.author | Ju, Young Ran | - |
dc.contributor.author | An, Seong Soo A. | - |
dc.contributor.author | Kim, Chi-Kyeong | - |
dc.date.available | 2020-02-29T05:45:48Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-07-27 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16276 | - |
dc.description.abstract | Misfolding of prion protein (PrP to PrPSc) can cause neurodegenerative prion diseases. As a glycosylphosphatidylinositol (GPI)-anchored membrane protein, the normal form of PrP (PrPC) can function as a receptor for ligands in the extracellular space. PrPC was suggested to be involved in memory, synaptic neuronal communication, and anti-oxidation as a neuroprotective agent. The recently identified interaction between PrPC and A beta(1-42) oligomers suggested another role for PrP as a receptor for A beta(1-42) oligomers, thereby influencing cytotoxicity and apoptosis. Here, the association between PrPC and A beta(1-42) oligomers was investigated by visualizing protein localization in neuronal cells by immunocytochemistry. A beta(1-42) oligomer-induced cytotoxicity was tested in respective expressions of PrPC by using mouse neuroblastoma-2a (N2a) cells, the prion protein over-expressed cells (L2-2B1), and a Prnp-null mouse hippocampal cell line (HpL 3-4). Moreover, apoptotic proteins such as caspase-8 were used to assess the effect of PrPC on A beta(1-42) oligomer-mediated apoptosis. In L2-2B1 and HpL 3-4 cells, the difference in the cytotoxicity of A beta(1-42) oligomers could be clearly distinguished. In addition, A beta(1-42) oligomers induced mitochondria dysfunction, reactive oxygen species (ROS) generation, and calcium influx PrPC-dependently. Apoptosis, related to mitochondria dysfunction, was further investigated to determine the cytotoxic pathway; the results suggest that PrPC could be involved in both the intrinsic and extrinsic apoptotic pathways. Finally, cells with abundant PrPC expression seemed to be more susceptible to A beta(1-42) oligomer toxicity, suggesting the importance of the level of PrPC expression in the induction of apoptosis. (C) 2012 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.subject | NEURONAL CELL-DEATH | - |
dc.subject | BETA OLIGOMERS | - |
dc.subject | DISEASE | - |
dc.subject | POTENTIATION | - |
dc.subject | CHANNELS | - |
dc.subject | BIOLOGY | - |
dc.title | The association between prion proteins and A beta(1-42) oligomers in cytotoxicity and apoptosis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000307369100003 | - |
dc.identifier.doi | 10.1016/j.bbrc.2012.06.056 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.424, no.2, pp.214 - 220 | - |
dc.identifier.scopusid | 2-s2.0-84864307099 | - |
dc.citation.endPage | 220 | - |
dc.citation.startPage | 214 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 424 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Hyeon, Jae Wook | - |
dc.contributor.affiliatedAuthor | Kim, Su Yeon | - |
dc.contributor.affiliatedAuthor | Park, Jun Sun | - |
dc.contributor.affiliatedAuthor | Choi, Bo Yeong | - |
dc.contributor.affiliatedAuthor | Lee, Sol Moe | - |
dc.contributor.affiliatedAuthor | Ju, Young Ran | - |
dc.contributor.affiliatedAuthor | Kim, Chi-Kyeong | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Prion | - |
dc.subject.keywordAuthor | A beta(1-42) oligomer | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Cell death | - |
dc.subject.keywordPlus | NEURONAL CELL-DEATH | - |
dc.subject.keywordPlus | BETA OLIGOMERS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | POTENTIATION | - |
dc.subject.keywordPlus | CHANNELS | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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