Inhibition of erythropoiesis by Smad6 in human cord blood hematopoietic stem cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Young-Ju | - |
dc.contributor.author | Shin, Ji-woong | - |
dc.contributor.author | Yoon, Jeong-Hwan | - |
dc.contributor.author | Oh, Il-Hwan | - |
dc.contributor.author | Lee, Soon-Pyo | - |
dc.contributor.author | Kim, Suk-Young | - |
dc.contributor.author | Park, Seok Hee | - |
dc.contributor.author | Mamura, Mizuko | - |
dc.date.available | 2020-02-29T05:45:55Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-07-13 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16281 | - |
dc.description.abstract | Bone morphogenetic proteins (BMPs) that belong to the transforming growth factor-beta (TGF-beta) superfamily cytokines, play crucial roles in hematopoiesis. However, roles of Smad6 in hematopoiesis remained unknown in contrast to the other inhibitory Smad (I-Smad), Smad7. Here we show that Smad6 inhibits erythropoiesis in human CD34(+) cord blood hematopoietic stem cells (HSCs). Smad6 was specifically expressed in CD34(+) cord blood HSCs, which was correlated with the expression of BMP2/4/6/7 and BMP type I receptor (BMPRI). BMP-specific receptor-regulated Smads (R-Smads), Smad1 and Smad5 in cooperation with Smad4 induced transcription of the Smad6 gene. Instead of affecting cell cycle, apoptosis, self-renewal, and stemness of CD34(+) cells. Smad6 knockdown enhanced, whereas Smad6 overexpression suppressed erythropoiesis in stem cell culture and colony formation assay. Consistently, Smad6 suppressed the expression of the genes essential for erythropoiesis, such as Kruppel-like factor 1 (erythroid) (KLF1/EKLF) and GATA binding protein 2 (GATA-2). Promoter analyses showed that Smad6 repressed Smad5/4-induced transcription of the Klf1 gene. Thus, our data suggest that Smad6 indirectly maintains stemness by preventing spontaneous erythropoiesis in HSCs. (C) 2012 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.subject | TGF-BETA SUPERFAMILY | - |
dc.subject | SELF-RENEWAL | - |
dc.subject | EMBRYONIC HEMATOPOIESIS | - |
dc.subject | RUNX1 ACTIVITY | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | RECEPTORS | - |
dc.subject | INDUCTION | - |
dc.subject | PROTEINS | - |
dc.subject | MESODERM | - |
dc.subject | BMP-4 | - |
dc.title | Inhibition of erythropoiesis by Smad6 in human cord blood hematopoietic stem cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000307032400023 | - |
dc.identifier.doi | 10.1016/j.bbrc.2012.06.031 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.423, no.4, pp.750 - 756 | - |
dc.identifier.scopusid | 2-s2.0-84863829184 | - |
dc.citation.endPage | 756 | - |
dc.citation.startPage | 750 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 423 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Kang, Young-Ju | - |
dc.contributor.affiliatedAuthor | Shin, Ji-woong | - |
dc.contributor.affiliatedAuthor | Yoon, Jeong-Hwan | - |
dc.contributor.affiliatedAuthor | Lee, Soon-Pyo | - |
dc.contributor.affiliatedAuthor | Kim, Suk-Young | - |
dc.contributor.affiliatedAuthor | Mamura, Mizuko | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Smad6 | - |
dc.subject.keywordAuthor | Human cord blood hematopoietic stem cells | - |
dc.subject.keywordAuthor | Erythropoiesis | - |
dc.subject.keywordAuthor | BMP | - |
dc.subject.keywordPlus | TGF-BETA SUPERFAMILY | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | EMBRYONIC HEMATOPOIESIS | - |
dc.subject.keywordPlus | RUNX1 ACTIVITY | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | MESODERM | - |
dc.subject.keywordPlus | BMP-4 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.