Smad3 regulates E-cadherin via miRNA-200 pathway
DC Field | Value | Language |
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dc.contributor.author | Ahn, S-M | - |
dc.contributor.author | Cha, J-Y | - |
dc.contributor.author | Kim, J. | - |
dc.contributor.author | Kim, D. | - |
dc.contributor.author | Trang, H. T. H. | - |
dc.contributor.author | Kim, Y-M | - |
dc.contributor.author | Cho, Y-H | - |
dc.contributor.author | Park, D. | - |
dc.contributor.author | Hong, S. | - |
dc.date.available | 2020-02-29T05:48:09Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16365 | - |
dc.description.abstract | To identify potential microRNA (miRNA) links between Smad3, a mediator of TGF-beta (transforming growth factor-beta) signaling, and E-cadherin, we characterized the miRNA profiles of two gastric cancer cell lines: SNU484-LPCX, which does not express Smad3, and SNU484-Smad3, in which Smad3 is overexpressed. We found that among differentially expressed miRNAs, miR-200 family members are overexpressed in SNU484-Smad3 cells. Subsequent studies, including analysis of the effects of silencing Smad3 in SNU484-Smad3 cells and a luciferase reporter assay, revealed that Smad3 directly binds to a Smad-binding element located in the promoter region of miR-200b/a, where it functions as a transcriptional activator. TGF-beta did not affect the regulatory role of Smad3 in transcription of miR-200 and expression of epithelial-mesenchymal transition markers. We conclude that Smad3 regulates, at the transcriptional level, miR-200 family members, which themselves regulate ZEB1 and ZEB2, known transcriptional repressors of E-cadherin, at the posttranscriptional level in a TGF-beta-independent manner. This represents a novel link between Smad3 and posttranscriptional regulation by miRNAs in epithelial-mesenchymal transition in gastric cancer cells. Oncogene (2012) 31, 3051-3059; doi: 10.1038/onc.2011.484; published online 24 October 2011 | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.relation.isPartOf | ONCOGENE | - |
dc.subject | GROWTH-FACTOR-BETA | - |
dc.subject | EPITHELIAL-MESENCHYMAL TRANSITION | - |
dc.subject | HUMAN GASTRIC-CANCER | - |
dc.subject | II RECEPTOR GENE | - |
dc.subject | TGF-BETA | - |
dc.subject | MIR-200 FAMILY | - |
dc.subject | MICROSATELLITE INSTABILITY | - |
dc.subject | COLORECTAL-CANCER | - |
dc.subject | REPRESSORS ZEB1 | - |
dc.subject | TUMOR INVASION | - |
dc.title | Smad3 regulates E-cadherin via miRNA-200 pathway | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000305705900004 | - |
dc.identifier.doi | 10.1038/onc.2011.484 | - |
dc.identifier.bibliographicCitation | ONCOGENE, v.31, no.25, pp.3051 - 3059 | - |
dc.identifier.scopusid | 2-s2.0-84862703901 | - |
dc.citation.endPage | 3059 | - |
dc.citation.startPage | 3051 | - |
dc.citation.title | ONCOGENE | - |
dc.citation.volume | 31 | - |
dc.citation.number | 25 | - |
dc.contributor.affiliatedAuthor | Ahn, S-M | - |
dc.contributor.affiliatedAuthor | Cha, J-Y | - |
dc.contributor.affiliatedAuthor | Kim, J. | - |
dc.contributor.affiliatedAuthor | Kim, D. | - |
dc.contributor.affiliatedAuthor | Trang, H. T. H. | - |
dc.contributor.affiliatedAuthor | Kim, Y-M | - |
dc.contributor.affiliatedAuthor | Cho, Y-H | - |
dc.contributor.affiliatedAuthor | Park, D. | - |
dc.contributor.affiliatedAuthor | Hong, S. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Smad3 | - |
dc.subject.keywordAuthor | miRNA-200 | - |
dc.subject.keywordAuthor | E-cadherin | - |
dc.subject.keywordAuthor | ZEB1/2 | - |
dc.subject.keywordAuthor | gastric cancer | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | EPITHELIAL-MESENCHYMAL TRANSITION | - |
dc.subject.keywordPlus | HUMAN GASTRIC-CANCER | - |
dc.subject.keywordPlus | II RECEPTOR GENE | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | MIR-200 FAMILY | - |
dc.subject.keywordPlus | MICROSATELLITE INSTABILITY | - |
dc.subject.keywordPlus | COLORECTAL-CANCER | - |
dc.subject.keywordPlus | REPRESSORS ZEB1 | - |
dc.subject.keywordPlus | TUMOR INVASION | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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