Increased Expression of Hypoxia-Inducible Factor-1 alpha and Connective Tissue Growth Factor Accompanied by Fibrosis in the Rat Testis of Varicocele
- Authors
- Paick, J. S.; Park, K.; Kim, S. W.; Park, J. W.; Kim, J. J.; Kim, M. S.; Park, J. Y.
- Issue Date
- May-2012
- Publisher
- ENE EDICIONES SL
- Keywords
- Alpha subunit; Connective tissue growth factor; Fibrosis; Hypoxia inducible factor 1; Varicocele
- Citation
- ACTAS UROLOGICAS ESPANOLAS, v.36, no.5, pp.282 - 288
- Journal Title
- ACTAS UROLOGICAS ESPANOLAS
- Volume
- 36
- Number
- 5
- Start Page
- 282
- End Page
- 288
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16409
- DOI
- 10.1016/j.acuroe.2012.08.005
- ISSN
- 0210-4806
- Abstract
- Objective: We investigated hypoxia inducible factor-1 alpha (HIF-1 alpha), connective tissue growth factor (CTGF) expression and fibrosis in the testis of rats with surgically induced varicocele. Material and methods: A total of 47 adult male Sprague-Dawley rats were arranged in 3 groups, namely group 1 (varicocele operation 4 weeks ago, n = 10; sham operation 4 weeks ago, n = 5), group 2 (8 weeks, n = 11; n = 5), and group 3 (12 weeks, n = 11; n = 5). The rats in every group underwent bilateral orchiectomy 4, 8, and 12 weeks after the operations, respectively. HIF-1 alpha and CTGF expression of both testes in group 3 were studied by real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Fibrotic change was assessed by quantitative image analysis. Results: HIF-1 alpha mRNA expression in testes tissues in varicocele operation and sham controls showed no significant differences in RT-PCR. However, CTGF mRNA expressions in left testes were found to be significantly different between varicocele operation and sham controls. HIFI a staining was present in both testes of all specimens and CTGF staining was present in 10 left and 8 right testes of 11 specimens. However HIF-1 alpha. and CTGF staining were absent in control group. There were significant fibrotic changes of both testes in groups 2 and 3. There were significant differences in fibrotic change along the durations of surgical varicocele. Conclusions: This study reveals that experimental varicocele in the rat is associated with HIF-1 alpha. and CTGF expression and it is accompanied by fibrotic change in the testis. (C) 2011 AEU. Published by Elsevier Espana, S.L. All rights reserved.
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