Genome Organization of Temperate Phage 11143 from Emetic Bacillus cereus NCTC11143
- Authors
- Lee, Young-Duck; Park, Jong-Hyun
- Issue Date
- May-2012
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- B cereus; temperate phage; DNA sequence; sequence analysis
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.22, no.5, pp.649 - 653
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 22
- Number
- 5
- Start Page
- 649
- End Page
- 653
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16422
- DOI
- 10.4014/jmb.1110.10065
- ISSN
- 1017-7825
- Abstract
- A temperate phage was isolated from emetic Bacillus cereus NCTC 11143 by mitomycin C and characterized by transmission electron microscopy and DNA and protein analyses. Whole genome sequencing of Bacillus phage 11143 was performed by GS-FLX. The phage has a dsDNA genome of 39,077 bp and a 35% G+C content. Bioinformatic analysis of the phage genome revealed 49 putative ORFs involved in replication, morphogenesis, DNA packaging, lysogeny, and host lysis. Bacillus phage 11143 could be classified as a member of the Siphoviridae family by morphology and genome structure. Genomic comparisons at the DNA and protein levels revealed homologous genetic modules with patterns and morphogenesis proteins similar to those of other Bacillus phages. Thus, Bacillus phages might have a mosaic genetic relationship.
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Collections - 바이오나노대학 > 식품생물공학과 > 1. Journal Articles
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