Electrochemical performance of gold nanoparticle-cytochrome c hybrid interface for H2O2 detection
DC Field | Value | Language |
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dc.contributor.author | Yagati, Ajay Kumar | - |
dc.contributor.author | Lee, Taek | - |
dc.contributor.author | Min, Junhong | - |
dc.contributor.author | Choi, Jeong-Woo | - |
dc.date.available | 2020-02-29T06:42:29Z | - |
dc.date.created | 2020-02-05 | - |
dc.date.issued | 2012-04-01 | - |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16448 | - |
dc.description.abstract | Here, we describe the formation of a hybrid biointerface consisting of gold nanoparticle (AuNP) and cytochrome c (cyt c) on indium tin oxide (ITO) electrodes using a two-step immobilization procedure. The Au nanoparticles were attached to the ITO electrodes by 3-mercaptopropyl trimethoxysilane (3-MPTMS). The electrode was then incubated with 11-mercapunclecanoic acid (11-MIJA) and the nanoparticles were activated to allow for coupling to cyt c. This process resulted in the formation of the AuNP/cyt c hybrid on the ITO electrode. The ITO/AuNP/cyt c substrate surfaces were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM) and X-ray diffraction analysis (XRD), and cyclic voltammetry (CV) techniques. Further analysis regarding the surface roughness properties of ITO, ITO/AuNP and ITO/AuNP/cyt c were also performed. The ITO/AuNP/cyt c immobilized ITO electrode displayed a pair of well-defined redox peaks (E-pa at 0.09 V and E-pc at 0.02V) at pH 7.0 in HEPES buffer solution. Differential pulse voltammetry (DPV) and amperometric i-t measurements on the modified electrode showed a linear response after the addition of hydrogen peroxide (H2O2). The developed electrode sensor had an electron transfer rate constant (k(s)) of 0.69 s(-1) with a detection limit of 0.5 mu M. The results of this study suggest that the hybrid layers were well fabricated on the ITO surface and the developed ITO/AuNP/cyt c electrode displayed an excellent electrocatalytic response for the detection of H2O2. (C) 2011 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.subject | DIRECT ELECTRON-TRANSFER | - |
dc.subject | HYDROGEN-PEROXIDE BIOSENSOR | - |
dc.subject | MULTIWALL CARBON NANOTUBES | - |
dc.subject | SURFACE-PLASMON RESONANCE | - |
dc.subject | HORSERADISH-PEROXIDASE | - |
dc.subject | AMPEROMETRIC DETERMINATION | - |
dc.subject | CHARGE INJECTION | - |
dc.subject | PROTEIN CHIP | - |
dc.subject | THIN-FILMS | - |
dc.subject | HEMOGLOBIN | - |
dc.title | Electrochemical performance of gold nanoparticle-cytochrome c hybrid interface for H2O2 detection | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000300859000023 | - |
dc.identifier.doi | 10.1016/j.colsurfb.2011.11.035 | - |
dc.identifier.bibliographicCitation | COLLOIDS AND SURFACES B-BIOINTERFACES, v.92, pp.161 - 167 | - |
dc.identifier.scopusid | 2-s2.0-84856107096 | - |
dc.citation.endPage | 167 | - |
dc.citation.startPage | 161 | - |
dc.citation.title | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.citation.volume | 92 | - |
dc.contributor.affiliatedAuthor | Min, Junhong | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Cytochrome c | - |
dc.subject.keywordAuthor | Gold nanoparticle | - |
dc.subject.keywordAuthor | Atomic force microscopy | - |
dc.subject.keywordAuthor | Cyclic voltammetry | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordPlus | DIRECT ELECTRON-TRANSFER | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE BIOSENSOR | - |
dc.subject.keywordPlus | MULTIWALL CARBON NANOTUBES | - |
dc.subject.keywordPlus | SURFACE-PLASMON RESONANCE | - |
dc.subject.keywordPlus | HORSERADISH-PEROXIDASE | - |
dc.subject.keywordPlus | AMPEROMETRIC DETERMINATION | - |
dc.subject.keywordPlus | CHARGE INJECTION | - |
dc.subject.keywordPlus | PROTEIN CHIP | - |
dc.subject.keywordPlus | THIN-FILMS | - |
dc.subject.keywordPlus | HEMOGLOBIN | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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