Galangin induces apoptosis in gastric cancer cells via regulation of ubiquitin carboxy-terminal hydrolase isozyme L1 and glutathione S-transferase P
DC Field | Value | Language |
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dc.contributor.author | Kim, Deuk Ae | - |
dc.contributor.author | Jeon, Young Keul | - |
dc.contributor.author | Nam, Myeong Jin | - |
dc.date.available | 2020-02-29T06:45:20Z | - |
dc.date.created | 2020-02-05 | - |
dc.date.issued | 2012-03 | - |
dc.identifier.issn | 0278-6915 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16567 | - |
dc.description.abstract | Galangin has been shown to have anti-cancer property against several types of cancer cells. Many studies have described the anti-oxidant and apoptotic effects of galangin. However, the mechanism of galangin-induced apoptosis has not yet been studied for human gastric cancer cells. We investigated galangin-induced apoptosis of human gastric cancer SNU-484 cells. Galangin inhibited proliferation of SNU-484 cells in a dose- and time-dependent manner. The results showed that galangin significantly decreased the viability of SNU-484 cells at 50-200 mu M for 24 h and 48 h. Galangin-induced cell death was characterized with the changes in cell morphology. DNA fragmentation, cell cycle, activation of caspase-3/-9, poly (ADP-ribose) polymerase (PARP) cleavage, and expression of MAP kinase such as ERK1/2 and JNK. For identification of proteins potentially involved in apoptosis, a two-dimensional electrophoresis was employed. Proteomic analysis showed that several proteins were associated with anti-cancer properties of galangin. Of particular interest, these proteins included ubiquitin carboxy-terminal hydrolase isozyme L1 (Uch-L1) and glutathione S-transferase P (GSTP), which are involved in apoptosis of SNU-484 cells. Western blot analysis confirmed up-regulation of Uch-L1 and down-regulation of GSTP following galangin treatment. Our results suggest that Uch-L1 and GSTP be involved in galangin-induced apoptosis in human gastric cancer SNU-484 cells. (c) 2011 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.relation.isPartOf | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.subject | PROLIFERATION | - |
dc.subject | ANTIOXIDANT | - |
dc.subject | FLAVONOIDS | - |
dc.subject | SYSTEM | - |
dc.title | Galangin induces apoptosis in gastric cancer cells via regulation of ubiquitin carboxy-terminal hydrolase isozyme L1 and glutathione S-transferase P | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000303284600036 | - |
dc.identifier.doi | 10.1016/j.fct.2011.11.039 | - |
dc.identifier.bibliographicCitation | FOOD AND CHEMICAL TOXICOLOGY, v.50, no.3-4, pp.684 - 688 | - |
dc.identifier.scopusid | 2-s2.0-84862784754 | - |
dc.citation.endPage | 688 | - |
dc.citation.startPage | 684 | - |
dc.citation.title | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.citation.volume | 50 | - |
dc.citation.number | 3-4 | - |
dc.contributor.affiliatedAuthor | Kim, Deuk Ae | - |
dc.contributor.affiliatedAuthor | Jeon, Young Keul | - |
dc.contributor.affiliatedAuthor | Nam, Myeong Jin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Flavonoid | - |
dc.subject.keywordAuthor | Galangin | - |
dc.subject.keywordAuthor | Gastric cancer | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Ubiquitin carboxy-terminal hydrolase | - |
dc.subject.keywordAuthor | isozyme L1 | - |
dc.subject.keywordAuthor | Glutathione S-transferase P | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | ANTIOXIDANT | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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