Apoptosis by aloe-emodin is mediated through down-regulation of calpain-2 and ubiquitin-protein ligase E3A in human hepatoma Huh-7 cells
DC Field | Value | Language |
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dc.contributor.author | Jeon, Won | - |
dc.contributor.author | Jeon, Young Keul | - |
dc.contributor.author | Nam, Myeong Jin | - |
dc.date.available | 2020-02-29T06:46:23Z | - |
dc.date.created | 2020-02-05 | - |
dc.date.issued | 2012-02 | - |
dc.identifier.issn | 1065-6995 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16608 | - |
dc.description.abstract | Natural flavonoids are associated with anti-proliferation of cancer growth. However, the antioxidant and anti-proliferation effects of AE (aloe-emodin) have not been well studied. We have investigated how AE affects the proliferation of hepatic hepatocellular carcinoma cells and exerts an anti-cancer effect. The cytotoxic effect of AE was demonstrated using an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay and Huh-7 cells were inhibited by AE treatment in both dose- and time-dependent manners. The IC50 level of AE was similar to 75 mu M. AE also has anti-proliferative effects via induction of DNA damage and apoptosis. 2-DE (two-dimensional electrophoresis) revealed that several proteins were related to the anti-cancer effects of AE. CAPN2 (calpain-2) and UBE3A (ubiquitin-protein ligase E3A), which are associated with the apoptosis signalling pathway, were verified by Western blotting. AE exhibited potent anti-proliferative effects on Huh-7 cells via down-regulation of CAPN2 and UBE3A. The findings support the possibility of AE being a chemopreventative agent. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PORTLAND PRESS LTD | - |
dc.relation.isPartOf | CELL BIOLOGY INTERNATIONAL | - |
dc.subject | CANCER-CELLS | - |
dc.subject | DNA-DAMAGE | - |
dc.subject | PATHWAY | - |
dc.subject | P53 | - |
dc.subject | EXPRESSION | - |
dc.subject | FLAVONOIDS | - |
dc.subject | QUERCETIN | - |
dc.subject | E6 | - |
dc.title | Apoptosis by aloe-emodin is mediated through down-regulation of calpain-2 and ubiquitin-protein ligase E3A in human hepatoma Huh-7 cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000300274500006 | - |
dc.identifier.doi | 10.1042/CBI20100723 | - |
dc.identifier.bibliographicCitation | CELL BIOLOGY INTERNATIONAL, v.36, no.2, pp.163 - 167 | - |
dc.identifier.scopusid | 2-s2.0-84859221153 | - |
dc.citation.endPage | 167 | - |
dc.citation.startPage | 163 | - |
dc.citation.title | CELL BIOLOGY INTERNATIONAL | - |
dc.citation.volume | 36 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Jeon, Won | - |
dc.contributor.affiliatedAuthor | Jeon, Young Keul | - |
dc.contributor.affiliatedAuthor | Nam, Myeong Jin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | aloe emodin | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | calpain-2 | - |
dc.subject.keywordAuthor | hepatocellular carcinoma | - |
dc.subject.keywordAuthor | Huh-7 cells | - |
dc.subject.keywordAuthor | ubiquitin-protein ligase E3A | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | QUERCETIN | - |
dc.subject.keywordPlus | E6 | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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