Exendin-4 Protects Against Sulfonylurea-Induced beta-Cell Apoptosis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ju-Young | - |
dc.contributor.author | Lim, Dong-Mee | - |
dc.contributor.author | Park, Hyung-Seo | - |
dc.contributor.author | Moon, Chan-Il | - |
dc.contributor.author | Choi, Kyung-Jin | - |
dc.contributor.author | Lee, Seong-Kyu | - |
dc.contributor.author | Baik, Haing-Woon | - |
dc.contributor.author | Park, Keun-Young | - |
dc.contributor.author | Kim, Byung-Joon | - |
dc.date.available | 2020-02-29T06:47:59Z | - |
dc.date.created | 2020-02-05 | - |
dc.date.issued | 2012-01 | - |
dc.identifier.issn | 1347-8613 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16663 | - |
dc.description.abstract | Sulfonylurea is one of the commonly used anti-diabetic drugs that stimulate insulin secretion from beta-cells. Despite their glucose lowering effects in type 2 diabetes mellitus, long-term treatment brought on secondary failure characterized by beta-cell exhaustion and apoptosis. ER stress induced by Ca2+ depletion in endoplasmic reticulum (ER) is speculated be one of the causes of secondary failure, but it remains unclear. Glucagon like peptide-1 (GLP-1) has anti-apoptotic effects in beta-cells after the induction of oxidative and ER stress. In this study, we examined the antiapoptotic action of a GLP-1 analogue in beta-cell lines and islets against ER stress induced by chronic treatment of sulfonylurea. HIT-T15 and dispersed islet cells were exposed to glibenclamide for 48 h, and apoptosis was evaluated using Annexin/PI flow cytometry. Expression of the ER stress related molecules and sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 2/3 was determined by real-time PCR and western blot analysis. Chronic exposure to glibenclamide increased apoptosis by depletion of ER Ca2+ concentration through reduced expression of SERCA 2/3. Pretreatment with Exendin-4 had an anti-apoptotic role through ER stress modulation and ER Ca2+ replenishing by SERCA restoration. These findings will further the understanding of one cause of gliben-clamide-induced-beta-cell loss and therapeutic availability of GLP-1 based drugs in secondary failure by sulfonylurea during treatment of diabetes. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | JAPANESE PHARMACOLOGICAL SOC | - |
dc.relation.isPartOf | JOURNAL OF PHARMACOLOGICAL SCIENCES | - |
dc.subject | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject | GLUCAGON-LIKE PEPTIDE-1 | - |
dc.subject | DIABETES-MELLITUS | - |
dc.subject | PROINSULIN LEVELS | - |
dc.subject | ISLETS | - |
dc.subject | DEATH | - |
dc.subject | CA2+ | - |
dc.subject | ATP | - |
dc.subject | GLIBENCLAMIDE | - |
dc.subject | CA2+-ATPASE | - |
dc.title | Exendin-4 Protects Against Sulfonylurea-Induced beta-Cell Apoptosis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000299860500007 | - |
dc.identifier.doi | 10.1254/jphs.11072FP | - |
dc.identifier.bibliographicCitation | JOURNAL OF PHARMACOLOGICAL SCIENCES, v.118, no.1, pp.65 - 74 | - |
dc.identifier.scopusid | 2-s2.0-84863031805 | - |
dc.citation.endPage | 74 | - |
dc.citation.startPage | 65 | - |
dc.citation.title | JOURNAL OF PHARMACOLOGICAL SCIENCES | - |
dc.citation.volume | 118 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Moon, Chan-Il | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Joon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | endoplasmic reticulum (ER) calcium depletion | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | ER stress | - |
dc.subject.keywordAuthor | glucagon like peptide-1(GLP-1) | - |
dc.subject.keywordAuthor | sulfonylurea | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject.keywordPlus | GLUCAGON-LIKE PEPTIDE-1 | - |
dc.subject.keywordPlus | DIABETES-MELLITUS | - |
dc.subject.keywordPlus | PROINSULIN LEVELS | - |
dc.subject.keywordPlus | ISLETS | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | CA2+ | - |
dc.subject.keywordPlus | ATP | - |
dc.subject.keywordPlus | GLIBENCLAMIDE | - |
dc.subject.keywordPlus | CA2+-ATPASE | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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