동충하초가 파골세포의 분화와 유전자 발현에 미치는 영향

Abstract

Objectives: This study was performed to evaluate effects of Cordyceps militaris(CM) on osteoclast differentiation and its related gene expression. Methods: We used mouse myeloid cells RAW 264.7 stimulated by receptor activator of nuclear factor kappa-B ligand(RANKL) to induce osteoclast differentiation. There are four groups of which RAW 264.7 cells are not stimulated by RANKL (Normal), stimulated by RANKL without CM(Control), stimulated by RANKL with 0.1 ㎍/㎖ of CM(CM 0.1), stimulated by RANKL with 1 ㎍/㎖ of CM(CM 1). Osteoclastogenesis was measured by counting Tartrate-resistant acid phosphatase-positive multinucleated cells [TRAP(+) MNC]. RT-PCR was performed to evaluate the inhibitory effect of CM on gene expression(TRAP, AKT1, JNK1, NFATc1, c-Fos,MITF). Results:1. CM decreased the number of TRAP(+) osteoclast in RANKL-stimulated RAW 264.7 cell at the concentration of 0.1 ㎍/㎖ and 1 ㎍/㎖. 2. CM decreased the expression of TRAP in osteoclast at the concentration of 1 ㎍/㎖. 3. CM decreased the expression of AKT1, JNK1 in osteoclast at the concentration of 1 ㎍/㎖. 4. CM didn’t affect the expression of NFATc1, c-Fos, MITF in osteoclast. Conclusions: Cordyceps militaris has inhibitory effects on osteoclast differentiation and its related gene expression. These results suggest that Cordyceps militaris has a potential as a treatment of osteoporosis.