정공피 추출물이 CoCl2에 의해 발생한 Neuroblastoma Cell Line SK-N-MC 세포자멸사의 보호효과

Abstract

Objectives :Sorbus commixta cortex(SCC) has been traditionally used in Korea, China and Japan for the treatment of cough, asthma, and other bronchial disorders. Cobalt chloride(CoCl2) is used to induce hypoxic condition in vitro study. The purpose of this study was to evaluate the effects of SCC extract on CoCl2-induced apoptosis in human SK-N-MC neuroblastoma cells. Methods :In order to investigate whether SCC possesses a protective effect on CoCl2-induced cytotoxicity in neuronal cells, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI(4,6-diamidino-2-phenylindole) staining, TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) assay, DNA fragmentation assay, flow cytometry, DNA fragmentation assay, and Western blotting were performed on SK-N-MC neuroblastoma cells. Results :Cells treated with CoCl2 exhibited several apoptotic features, while cells pre-treated with SCC prior to CoCl2 exposure showed a decrease in the occurrence of apoptotic features. CoCl2 increased HIF-1α expression, however, SCC treatment decreased CoCl2-induced HIF-1α expression. The extract of SCC treatment suppressed Bax and caspase-3 expressions, and increased Bcl-2 expression in human neuroblastoma SK-N-MC cells. Conclusions :These results suggest that SCC may exert a protective effect on CoCl2-induced apoptosis in SK-N-MC neuroblastoma cells.