An application of electrostatic repulsion hydrophilic interaction chromatography in phospho- and glycoproteome profiling of epicardial adipose tissue in obesity mouse
DC Field | Value | Language |
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dc.contributor.author | Huyen Tran, T. | - |
dc.contributor.author | Hwang, I. | - |
dc.contributor.author | Park, J.-M. | - |
dc.contributor.author | Bum Kim, J. | - |
dc.contributor.author | Lee, H. | - |
dc.date.available | 2020-02-29T09:45:23Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 2233-4203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17506 | - |
dc.description.abstract | Phosphorylation and glycosylation are two of the most important and widespread post-translational modifications (PTMs) in an organism. Proteomics analysis of the PTMs has been challenged by low stoichiometry of the modified proteins and suppression effects by high abundance proteins, typically no-functional house-keeping proteins. In this study, a novel method was applied for not only isolating PTM peptides from intact peptides but also concurrently characterizing of glyco- and phospho-proteome using electrostatic repulsion hydrophilic interaction chromatography (ERLIC) packed with silica coated by cross-linked polyethyleneimine. For 2 mg tryptic digest of mouse proteome of epicardial adipose tissue with fat diet, 802 N-glycosy-lated peptides of 316 glycoproteins and 159 phosphorylated peptides of 75 phosphoproteins were identified using HPLC chip/ quadrupole time-of-flight (Q-TOF) tandem mass spectrometer. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.relation.isPartOf | Mass Spectrometry Letters | - |
dc.title | An application of electrostatic repulsion hydrophilic interaction chromatography in phospho- and glycoproteome profiling of epicardial adipose tissue in obesity mouse | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.doi | 10.5478/MSL.2012.3.2.39 | - |
dc.identifier.bibliographicCitation | Mass Spectrometry Letters, v.3, no.2, pp.39 - 42 | - |
dc.identifier.kciid | ART001912058 | - |
dc.identifier.scopusid | 2-s2.0-84878680109 | - |
dc.citation.endPage | 42 | - |
dc.citation.startPage | 39 | - |
dc.citation.title | Mass Spectrometry Letters | - |
dc.citation.volume | 3 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Huyen Tran, T. | - |
dc.contributor.affiliatedAuthor | Park, J.-M. | - |
dc.contributor.affiliatedAuthor | Lee, H. | - |
dc.type.docType | Letter | - |
dc.subject.keywordAuthor | ERLIC | - |
dc.subject.keywordAuthor | Glycoproteome enrichment | - |
dc.subject.keywordAuthor | Phosphoproteome enrichment | - |
dc.subject.keywordAuthor | PTMs | - |
dc.subject.keywordAuthor | Simultaneous enrichment | - |
dc.description.journalRegisteredClass | scopus | - |
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