Chemiluminescent enzyme-linked immunosorbent assay on a strip to detect Escherichia coli O157:H7
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Sojung | - |
dc.contributor.author | Min, Junhong | - |
dc.contributor.author | Kim, Young-Kee | - |
dc.date.available | 2020-02-29T09:47:11Z | - |
dc.date.created | 2020-02-05 | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0306-7319 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17556 | - |
dc.description.abstract | A fast and sensitive chemiluminescent enzyme-linked immunosorbent assay method to measure pathogenic bacteria, Escherichia coli O157:H7, on immuno-chromatographic membrane was studied. Non-specific binding of proteins on membrane strip was controlled to attain the best performance of immunosensor by optimising the composition of a running buffer. The specificity of the proposed immunostrip was confirmed by conducting experiments for four different microorganisms. A chemiluminescent signal could be successfully generated from a proposed immunostrip sensing system, and a significant change in the chemiluminescent light intensity with the concentration of target microbes was obtained. E. coli O157:H7 could be quantitatively measured in the range of 1.1 x 10(3)-1.1 x 10(7) CFU (colony forming units) mL(-1) within 16 min by using the developed chemiluminescent immunostrip. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY | - |
dc.subject | RAPID DETECTION | - |
dc.subject | LISTERIA-MONOCYTOGENES | - |
dc.subject | O157-H7 | - |
dc.subject | BIOSENSOR | - |
dc.subject | FOOD | - |
dc.subject | IMPEDANCE | - |
dc.subject | MEMBRANE | - |
dc.subject | BACTERIA | - |
dc.subject | SAMPLES | - |
dc.title | Chemiluminescent enzyme-linked immunosorbent assay on a strip to detect Escherichia coli O157:H7 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000302789100002 | - |
dc.identifier.doi | 10.1080/03067311003782682 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, v.92, no.6, pp.655 - 664 | - |
dc.identifier.scopusid | 2-s2.0-84859731643 | - |
dc.citation.endPage | 664 | - |
dc.citation.startPage | 655 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 92 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Min, Junhong | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | enzyme-linked immunosorbent assay (ELISA) | - |
dc.subject.keywordAuthor | chemiluminescence | - |
dc.subject.keywordAuthor | Escherichia coli O157:H7 | - |
dc.subject.keywordAuthor | chromatographic membrane strip | - |
dc.subject.keywordAuthor | immunosensor | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | LISTERIA-MONOCYTOGENES | - |
dc.subject.keywordPlus | O157-H7 | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | FOOD | - |
dc.subject.keywordPlus | IMPEDANCE | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | SAMPLES | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.