All-Trans Retinoic Acid Enhances Bacterial Flagellin-Stimulated Proinflammatory Responses in Human Monocyte THP-1 Cells by Upregulating CD14
DC Field | Value | Language |
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dc.contributor.author | Hoang, Thi Xoan | - |
dc.contributor.author | Jung, Jong Hyeok | - |
dc.contributor.author | Kim, Jae Young | - |
dc.date.available | 2020-03-03T06:48:30Z | - |
dc.date.created | 2020-02-24 | - |
dc.date.issued | 2019-12-27 | - |
dc.identifier.issn | 2314-6133 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17867 | - |
dc.description.abstract | All-trans retinoic acid (ATRA), an active form of vitamin A, exerts immunomodulatory functions. In this study, we examined the immune potentiating effect of ATRA on bacterial flagellin-induced NF-kappa B activation and proinflammatory cytokine production in human monocytic cell line THP-1. ATRA treatment significantly enhanced the flagellin-induced NF-kappa B/AP-1 activity in THP-1 via the RAR/RXR pathway. Similarly, ATRA enhanced the expression and production of TNF-alpha and IL-1 beta in THP-1 cells upon flagellin challenge. The cell surface expression of toll-like receptor 5 (TLR5), which is the receptor for bacterial flagellin, was significantly reduced by ATRA in a concentration- and time-dependent manner. To determine the mechanisms underlying the ATRA-enhanced immune response against bacterial flagellin despite the reduced cell surface expression of TLR5 in ATRA-treated THP-1, we examined the cell surface expression of CD14, which has been proposed to be a TLR co-receptor that enhances the response to microbial components. The cell surface expression of CD14 was significantly enhanced by ATRA treatment, especially in the presence of flagellin. Anti-CD14 antibody treatment prior to ATRA and flagellin treatments completely abolished ATRA-enhanced TNF-alpha and IL-1 beta production. Our results suggest that ATRA enhances flagellin-stimulated proinflammatory responses in human monocyte THP-1 cells by upregulating CD14 in a RAR/RXR-dependent manner. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | HINDAWI LTD | - |
dc.relation.isPartOf | BIOMED RESEARCH INTERNATIONAL | - |
dc.title | All-Trans Retinoic Acid Enhances Bacterial Flagellin-Stimulated Proinflammatory Responses in Human Monocyte THP-1 Cells by Upregulating CD14 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000505936800007 | - |
dc.identifier.doi | 10.1155/2019/8059312 | - |
dc.identifier.bibliographicCitation | BIOMED RESEARCH INTERNATIONAL, v.2019 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85077941063 | - |
dc.citation.title | BIOMED RESEARCH INTERNATIONAL | - |
dc.citation.volume | 2019 | - |
dc.contributor.affiliatedAuthor | Hoang, Thi Xoan | - |
dc.contributor.affiliatedAuthor | Jung, Jong Hyeok | - |
dc.contributor.affiliatedAuthor | Kim, Jae Young | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | FACTOR-KAPPA-B | - |
dc.subject.keywordPlus | CUTTING EDGE | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | NEUROBLASTOMA | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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