Upregulation of caveolin-1 and its colocalization with cytokine receptors contributes to beta cell apoptosis
DC Field | Value | Language |
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dc.contributor.author | Bae, Gong Deuk | - |
dc.contributor.author | Park, Eun-Young | - |
dc.contributor.author | Kim, Kyong | - |
dc.contributor.author | Jang, Se-Eun | - |
dc.contributor.author | Jun, Hee-Sook | - |
dc.contributor.author | Oh, Yoon Sin | - |
dc.date.available | 2020-03-03T07:44:00Z | - |
dc.date.created | 2020-02-24 | - |
dc.date.issued | 2019-11-14 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17973 | - |
dc.description.abstract | Caveolin-1 (cav-1), the principal structural and signalling protein of caveolae, is implicated in various signalling events, including apoptotic cell death in type 2 diabetes. However, the precise role of beta cells in apoptosis has not been clearly defined. In this study, we investigated the involvement of cav-1 in cytokine-induced beta cell apoptosis and its underlying mechanisms in the rat beta cell line, INS-1 and isolated islets. Treatment of cytokine mixture (CM, TNF alpha + IL-1 beta) significantly increased the mRNA and protein expression of cav-1, and resulting in increased formation of caveolae. We found that IL-1 receptor 1 and TNF receptor localized to plasma membrane lipid rafts in the control cells and CM treatment recruited these receptors to the caveolae domain. After cav-1 siRNA transfection, CM-dependent NF-kappa B activation was reduced and consequently downregulated the mRNA expression of iNOS and IL-1 beta. Finally, decreased cell viability by CM treatment was ameliorated in both INS-1 cells and isolated islets treated with cav-1 siRNA. These results suggest that increased cav-1 expression and recruitment of cytokine receptors into caveolae contribute to CM-induced beta cell apoptosis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | PANCREATIC-ISLETS | - |
dc.subject | INSULIN | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | GENES | - |
dc.subject | DEATH | - |
dc.subject | TNF | - |
dc.subject | ACTIVATION | - |
dc.subject | EXPRESSION | - |
dc.subject | PACLITAXEL | - |
dc.title | Upregulation of caveolin-1 and its colocalization with cytokine receptors contributes to beta cell apoptosis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000496415500009 | - |
dc.identifier.doi | 10.1038/s41598-019-53278-z | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.9 | - |
dc.identifier.scopusid | 2-s2.0-85075059299 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 9 | - |
dc.contributor.affiliatedAuthor | Bae, Gong Deuk | - |
dc.contributor.affiliatedAuthor | Jun, Hee-Sook | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | PANCREATIC-ISLETS | - |
dc.subject.keywordPlus | INSULIN | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | TNF | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PACLITAXEL | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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