Inhibitory Effects of Roseoside and Icariside E4 Isolated from a Natural Product Mixture (No-ap) on the Expression of Angiotensin II Receptor 1 and Oxidative Stress in Angiotensin II-Stimulated H9C2 Cells
DC Field | Value | Language |
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dc.contributor.author | Hong, Eun Young | - |
dc.contributor.author | Kim, Tae Yang | - |
dc.contributor.author | Hong, Gwan Ui | - |
dc.contributor.author | Kang, Hanna | - |
dc.contributor.author | Lee, Jung-Yun | - |
dc.contributor.author | Park, Jae Yeo | - |
dc.contributor.author | Kim, Se-Chan | - |
dc.contributor.author | Kim, Young Ho | - |
dc.contributor.author | Chung, Myung-Hee | - |
dc.contributor.author | Kwon, Young-In | - |
dc.contributor.author | Ro, Jai Youl | - |
dc.date.available | 2020-02-27T04:41:40Z | - |
dc.date.created | 2020-02-05 | - |
dc.date.issued | 2019-02-01 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1856 | - |
dc.description.abstract | Hypertension is a major risk factor for the development of cardiovascular diseases. This study aimed to elucidate whether the natural product mixture No-ap (NA) containing Pine densiflora, Annona muricate, and Monordica charantia, or its single components have inhibitory effects on hypertension-related molecules in Angiotensin II (Ang II)-stimulated H9C2 cells. Individual functional components were isolated and purified from NA using various columns and solvents, and then their structures were analyzed using ESI-MS, H-1-NMR, and H-13-NMR spectra. H9C2 cells were stimulated with 300 nM Ang II for 7 h. NA, telmisartan, ginsenoside, roseoside (Roseo), icariside E4 (IE4), or a combination of two components (Roseo and IE4) were administered to the cells 1 h before Ang II stimulation. The expression and activity of hypertension-related molecules or oxidative molecules were determined using RT-PCR, western blot, and ELISA. Ang II stimulation increased the expression of Ang II receptor 1 (AT1), tumor necrosis factor-alpha (TNF-alpha), monocyte chemoattractant protein-1 (MCP-1), tumor growth factor-beta (TGF-beta) mRNA, and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and the levels of hydrogen peroxide (H2O2) and superoxide anion (center dot O-2(-)) and reduced anti-oxidant enzyme activity. NA significantly improved the expression or activities of all hypertension-related molecules altered in Ang II-stimulated cells. Roseo or IE4 pretreatment either decreased or increased the expression or activities of all hypertension-related molecules similar to NA, but to a lesser extent. The pretreatment with a combination of Roseo and IE4 (1:1) either decreased or increased the expression of all hypertension-related molecules, compared to each single component, revealing a synergistic action of the two compounds. Thus, the combination of single components could exert promising anti-hypertensive effects similar to NA, which should be examined in future animal and clinical studies. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | MOLECULES | - |
dc.subject | CARDIAC-HYPERTROPHY | - |
dc.subject | NADPH OXIDASE | - |
dc.subject | NITRIC-OXIDE | - |
dc.subject | HYPERTENSION | - |
dc.subject | NEEDLES | - |
dc.subject | CONSTITUENTS | - |
dc.subject | INFLAMMATION | - |
dc.subject | GLYCOSIDES | - |
dc.subject | NEOLIGNAN | - |
dc.subject | RELEASE | - |
dc.title | Inhibitory Effects of Roseoside and Icariside E4 Isolated from a Natural Product Mixture (No-ap) on the Expression of Angiotensin II Receptor 1 and Oxidative Stress in Angiotensin II-Stimulated H9C2 Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000458934000038 | - |
dc.identifier.doi | 10.3390/molecules24030414 | - |
dc.identifier.bibliographicCitation | MOLECULES, v.24, no.3 | - |
dc.identifier.scopusid | 2-s2.0-85060544664 | - |
dc.citation.title | MOLECULES | - |
dc.citation.volume | 24 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Chung, Myung-Hee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | cardiomyocytes (H9C2 cells) | - |
dc.subject.keywordAuthor | angiotensin II | - |
dc.subject.keywordAuthor | hypertension | - |
dc.subject.keywordAuthor | No-ap (natural product mixture) | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | roseoside | - |
dc.subject.keywordAuthor | icariside E4 | - |
dc.subject.keywordPlus | CARDIAC-HYPERTROPHY | - |
dc.subject.keywordPlus | NADPH OXIDASE | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | HYPERTENSION | - |
dc.subject.keywordPlus | NEEDLES | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | GLYCOSIDES | - |
dc.subject.keywordPlus | NEOLIGNAN | - |
dc.subject.keywordPlus | RELEASE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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