Colorimetric Detection of MPT64 Antibody Based on an Aptamer Adsorbed Magnetic Nanoparticles for Diagnosis of Tuberculosis
DC Field | Value | Language |
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dc.contributor.author | Cheon, Hong Jae | - |
dc.contributor.author | Lee, Sang Min | - |
dc.contributor.author | Kim, Seong-Rok | - |
dc.contributor.author | Shin, Ho Yun | - |
dc.contributor.author | Seo, Yiel Hea | - |
dc.contributor.author | Cho, Yong Kyun | - |
dc.contributor.author | Lee, Sang Pyo | - |
dc.contributor.author | Kim, Moon Il | - |
dc.date.available | 2020-02-27T04:41:47Z | - |
dc.date.created | 2020-02-05 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1873 | - |
dc.description.abstract | We have developed a colorimetric biosensing system for the detection of antibody against MPT64, a protein secreted by Mycobacterium tuberculosis, using aptamer DNA adsorbed Fe3O4 magnetic nanoparticles (MNPs) for diagnosis of tuberculosis (TB). In this system, MNPs were first incubated with single stranded (ss) DNA-type aptamer having a high affinity toward target antibody against MPT64 (anti-MPT64), resulting in quick inhibition of the peroxidase-like activity of MNPs via the adsorption of aptamer on the surface of MNPs. By the addition of sample solutions containing anti-MPT64, aptamer bound on the surface of MNPs would strongly interact with free anti-MPT64 and be detached from the MNPs, thereby increasing the available surface area of the MNPs and consequently yielding enhanced peroxidase activity. Using this strategy, target anti-MPT64 was successfully detected by displaying increased colorimetric intensities from the higher oxidation of employed peroxidase substrate, 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) (ABTS). Based on these results, we anticipate that aptamer adsorbed MNPs can serve as a potent probe system for the detection of clinically important target molecules. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.relation.isPartOf | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.title | Colorimetric Detection of MPT64 Antibody Based on an Aptamer Adsorbed Magnetic Nanoparticles for Diagnosis of Tuberculosis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000448031900002 | - |
dc.identifier.doi | 10.1166/jnn.2019.15905 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.19, no.2, pp.622 - 626 | - |
dc.citation.endPage | 626 | - |
dc.citation.startPage | 622 | - |
dc.citation.title | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.volume | 19 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Cheon, Hong Jae | - |
dc.contributor.affiliatedAuthor | Lee, Sang Min | - |
dc.contributor.affiliatedAuthor | Kim, Seong-Rok | - |
dc.contributor.affiliatedAuthor | Shin, Ho Yun | - |
dc.contributor.affiliatedAuthor | Seo, Yiel Hea | - |
dc.contributor.affiliatedAuthor | Cho, Yong Kyun | - |
dc.contributor.affiliatedAuthor | Lee, Sang Pyo | - |
dc.contributor.affiliatedAuthor | Kim, Moon Il | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Tuberculosis | - |
dc.subject.keywordAuthor | MPT64 Antibody | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | Magnetic Nanoparticles | - |
dc.subject.keywordAuthor | Peroxidase Mimic | - |
dc.subject.keywordAuthor | Colorimetric Biosensors | - |
dc.subject.keywordPlus | MESOPOROUS CARBON | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | OXIDASE | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | TB | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.description.journalRegisteredClass | scie | - |
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