Glucose oxidase-copper hybrid nanoflowers embedded with magnetic nanoparticles as an effective antibacterial agent
DC Field | Value | Language |
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dc.contributor.author | Lee I. | - |
dc.contributor.author | Cheon H.J. | - |
dc.contributor.author | Adhikari M.D. | - |
dc.contributor.author | Tran T.D. | - |
dc.contributor.author | Yeon K.-M. | - |
dc.contributor.author | Kim M.I. | - |
dc.contributor.author | Kim J. | - |
dc.date.available | 2020-03-03T12:42:53Z | - |
dc.date.created | 2020-02-24 | - |
dc.date.issued | 2020-07 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/19462 | - |
dc.description.abstract | Bacterial contamination causes various problems ranging from bacterial infection to biofouling. As an effective and non-toxic agent for bacterial de-contamination, glucose oxidase (GOx)-copper hybrid nanoflowers embedded with amine-functionalized magnetic nanoparticles (NH2-MNPs), called ‘MNP-GOx NFs’, are developed. Positively-charged NH2-MNPs and negatively-charged GOx molecules are first interacted via electrostatic attraction which can be controlled by changing the buffer pH, and the follow-up addition of copper(II) sulfate leads to blooming of nanoflowers (MNP-GOx NFs) after incubation at room temperature for 3 days. MNP-GOx NFs show effective antibacterial activity by generating H2O2 from GOx-catalyzed glucose oxidation. For example, 99.9% killings of Staphylococcus aureus and Escherichia coli are achieved after 3 h treatment of 106/mL cells with 0.2 and 3.0 mg/mL MNP-GOx NFs, respectively, revealing that Gram-positive S. aureus with mono-layer membrane system is more vulnerable to the treatment of MNP-GOx NFs than Gram-negative E. coli with two-layer membrane system. MNP-GOx NFs can maintain 97% of bactericidal activity even after recycled uses by magnetic separation for eight times iterative bacterial killings. Finally, MNP-GOx NFs are employed for the fabrication of antibacterial gauzes. MNP-GOx NFs have also opened up a great potential for their applications in biosensors, biofuel cells and bioconversion as well as bacterial de-contamination. © 2019 | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isPartOf | International Journal of Biological Macromolecules | - |
dc.title | Glucose oxidase-copper hybrid nanoflowers embedded with magnetic nanoparticles as an effective antibacterial agent | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000536122500157 | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2019.11.129 | - |
dc.identifier.bibliographicCitation | International Journal of Biological Macromolecules, v.155, pp.1520 - 1531 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85075899016 | - |
dc.citation.endPage | 1531 | - |
dc.citation.startPage | 1520 | - |
dc.citation.title | International Journal of Biological Macromolecules | - |
dc.citation.volume | 155 | - |
dc.contributor.affiliatedAuthor | Cheon H.J. | - |
dc.contributor.affiliatedAuthor | Tran T.D. | - |
dc.contributor.affiliatedAuthor | Kim M.I. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Antibacterial activity | - |
dc.subject.keywordAuthor | Enzyme-inorganic hybrid nanoflowers | - |
dc.subject.keywordAuthor | Glucose oxidase | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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