Small molecule target identification using photo-affinity chromatography
DC Field | Value | Language |
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dc.contributor.author | Seo, Seung-Yong | - |
dc.contributor.author | Corson, Timothy W. | - |
dc.date.available | 2020-03-03T12:44:32Z | - |
dc.date.created | 2020-02-24 | - |
dc.date.issued | 2019-03 | - |
dc.identifier.issn | 0076-6879 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/19519 | - |
dc.description.abstract | Identification of the protein targets of bioactive small molecules is a routine challenge in chemical biology and phenotype-based drug discovery. Recent years have seen an explosion of approaches to meeting this challenge, but the traditional method of affinity pulldowns remains a practical choice in many contexts. This technique can be used as long as an affinity probe can be synthesized, usually with a crosslinking moiety to enable photo-affinity pulldowns. It can be applied to varied tissue types and can be performed with minimal specialized equipment. Here, we provide our protocol for photo-affinity pulldown experiments, with notes on making this method generally applicable to varied target identification challenges. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ACADEMIC PRESS INC | - |
dc.relation.isPartOf | CHEMICAL AND SYNTHETIC BIOLOGY APPROACHES TO UNDERSTAND CELLULAR FUNCTIONS - PT B | - |
dc.title | Small molecule target identification using photo-affinity chromatography | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000500350700017 | - |
dc.identifier.doi | 10.1016/bs.mie.2019.02.028 | - |
dc.identifier.bibliographicCitation | CHEMICAL AND SYNTHETIC BIOLOGY APPROACHES TO UNDERSTAND CELLULAR FUNCTIONS - PT B, v.622, pp.347 - 374 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85062810637 | - |
dc.citation.endPage | 374 | - |
dc.citation.startPage | 347 | - |
dc.citation.title | CHEMICAL AND SYNTHETIC BIOLOGY APPROACHES TO UNDERSTAND CELLULAR FUNCTIONS - PT B | - |
dc.citation.volume | 622 | - |
dc.contributor.affiliatedAuthor | Seo, Seung-Yong | - |
dc.type.docType | Review; Book Chapter | - |
dc.subject.keywordAuthor | Biotin | - |
dc.subject.keywordAuthor | Click chemistry | - |
dc.subject.keywordAuthor | Inhibitor | - |
dc.subject.keywordAuthor | Mass spectrometry | - |
dc.subject.keywordAuthor | Natural product | - |
dc.subject.keywordAuthor | Off-target | - |
dc.subject.keywordAuthor | Photoreactive group | - |
dc.subject.keywordAuthor | Small molecule | - |
dc.subject.keywordAuthor | Synthetic chemistry | - |
dc.subject.keywordAuthor | Target identification | - |
dc.subject.keywordPlus | BIOLOGICAL EVALUATION | - |
dc.subject.keywordPlus | THERAPEUTIC TARGET | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | PROTEOMICS | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | DISCOVERY | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | BINDS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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