Simple and Label-Free Liquid Crystal-based Optical Sensor for Highly Sensitive and Selective Endotoxin Detection by Aptamer Binding and Separation
DC Field | Value | Language |
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dc.contributor.author | An, Zongfu | - |
dc.contributor.author | Jang, Chang-Hyun | - |
dc.date.available | 2020-02-27T04:42:26Z | - |
dc.date.created | 2020-02-05 | - |
dc.date.issued | 2019-01-31 | - |
dc.identifier.issn | 2365-6549 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1958 | - |
dc.description.abstract | Lipopolysaccharides (LPS), also commonly known as lipoglycans and endotoxins, can often accidentally contaminate recombinant protein therapeutics and bacteria-derived plasmid DNA vaccines. Since LPS can induce fever, hypotension, shock, and even death, their early and sensitive detection is necessary in relevant bioassays and for parenteral drug administration and/or biotherapeutics. In this study, an optical biosensor was developed using LPS-specific single-stranded DNA aptamers as LPS-selective probes. (3-Aminopropyl) triethoxysilane and glutaraldehyde were used as linkers to immobilize the LPS high-affinity aptamer on glass. Subsequently, each modification step was characterized by the optical responses obtained from liquid crystals using a polarized optical microscope. The sensor's ability to detect LPS was confirmed using a broad LPS detection range (5.5 pg/mL - 100 ng/mL). Despite the presence of plasmid DNA, RNA, and bovine serum albumin, the aptamer sensor showed high selectivity for LPS and could be regenerated for reuse at a low pH, thus, providing a promising option for detecting LPS in complex, low pH environments. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.relation.isPartOf | CHEMISTRYSELECT | - |
dc.subject | PHAGE DISPLAY | - |
dc.subject | DNA | - |
dc.subject | LIPOPOLYSACCHARIDE | - |
dc.subject | NANOPARTICLES | - |
dc.subject | PEPTIDES | - |
dc.subject | COCAINE | - |
dc.title | Simple and Label-Free Liquid Crystal-based Optical Sensor for Highly Sensitive and Selective Endotoxin Detection by Aptamer Binding and Separation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000457441400043 | - |
dc.identifier.doi | 10.1002/slct.201803774 | - |
dc.identifier.bibliographicCitation | CHEMISTRYSELECT, v.4, no.4, pp.1416 - 1422 | - |
dc.identifier.scopusid | 2-s2.0-85060893507 | - |
dc.citation.endPage | 1422 | - |
dc.citation.startPage | 1416 | - |
dc.citation.title | CHEMISTRYSELECT | - |
dc.citation.volume | 4 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | An, Zongfu | - |
dc.contributor.affiliatedAuthor | Jang, Chang-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | lipopolysaccharide | - |
dc.subject.keywordAuthor | liquid crystal | - |
dc.subject.keywordAuthor | 4-cyano-4 &apos | - |
dc.subject.keywordAuthor | -pentylbiphenyl | - |
dc.subject.keywordAuthor | optical biosensors | - |
dc.subject.keywordAuthor | polarized optical microscope | - |
dc.subject.keywordPlus | PHAGE DISPLAY | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | PEPTIDES | - |
dc.subject.keywordPlus | COCAINE | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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