Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1
DC Field | Value | Language |
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dc.contributor.author | Kim, Kem Ok | - |
dc.contributor.author | Lee, Dahae | - |
dc.contributor.author | Nguyen Tuan Hiep | - |
dc.contributor.author | Song, Ji Hoon | - |
dc.contributor.author | Lee, Hae-Jeung | - |
dc.contributor.author | Lee, Dongho | - |
dc.contributor.author | Kang, Ki Sung | - |
dc.date.available | 2020-02-27T04:42:43Z | - |
dc.date.created | 2020-02-05 | - |
dc.date.issued | 2019-01-01 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1990 | - |
dc.description.abstract | We investigated whether 14 phenolic compounds isolated from Artemisia argyi could prevent the apoptotic damage caused by iodixanol, an iodinated contrast agent, on LLC-PK1 cells. Iodixanol was used to induce cytotoxicity in LLC-PK1 cells. Apoptotic cell death was observed as the fluorescence intensity emitted by annexin V and Hoechst 33342 stains. Western blotting was used to detect specific proteins. Seven phenolic compounds protected against iodixanol-induced LLC-PK1 cell death in a concentration-dependent manner. Among them, methyl caffeate exerted the strongest protective effect, and co-treatment with 50 and 100 M methyl caffeate decreased intracellular reactive oxygen species elevated by 25 mg/mL iodixanol. In addition, the treatment of LLC-PK1 cells with iodixanol resulted in an increase in apoptotic cell death, which decreased by co-treatment with methyl caffeate. Iodixanol caused a cytotoxicity-related increase in the phosphorylation of extracellular-signal-regulated kinase, c-Jun N-terminal kinase, and P38; and a similar increase in the expression levels of kidney injury molecule-1 and cleaved caspase-3. However, the up-regulation of these proteins was reversed by co-treatment with methyl caffeate. These findings suggest that phenolic compounds isolated from A. argyi play an important role in protecting kidney epithelium cells against apoptotic damage caused by iodixanol. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | MOLECULES | - |
dc.subject | CHEMICAL-COMPOSITION | - |
dc.subject | ALPHA | - |
dc.subject | ACID | - |
dc.subject | INJURY | - |
dc.subject | CONSTITUENTS | - |
dc.subject | EXTRACT | - |
dc.subject | ACETYLCYSTEINE | - |
dc.subject | IDENTIFICATION | - |
dc.subject | TOCOPHEROL | - |
dc.subject | MOLECULE-1 | - |
dc.title | Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000457150200195 | - |
dc.identifier.doi | 10.3390/molecules24010195 | - |
dc.identifier.bibliographicCitation | MOLECULES, v.24, no.1 | - |
dc.identifier.scopusid | 2-s2.0-85059798386 | - |
dc.citation.title | MOLECULES | - |
dc.citation.volume | 24 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Song, Ji Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Hae-Jeung | - |
dc.contributor.affiliatedAuthor | Kang, Ki Sung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | contrast agent | - |
dc.subject.keywordAuthor | iodixanol | - |
dc.subject.keywordAuthor | cytotoxicity | - |
dc.subject.keywordAuthor | MAPKs | - |
dc.subject.keywordAuthor | caspase-3 | - |
dc.subject.keywordAuthor | kidney injury molecule-1 | - |
dc.subject.keywordPlus | CHEMICAL-COMPOSITION | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordPlus | ACETYLCYSTEINE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | TOCOPHEROL | - |
dc.subject.keywordPlus | MOLECULE-1 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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