Procyanidin C1 Activates the Nrf2/HO-1 Signaling Pathway to Prevent Glutamate-Induced Apoptotic HT22 Cell Death
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Ji Hoon | - |
dc.contributor.author | Lee, Hae-Jeung | - |
dc.contributor.author | Kang, Ki Sung | - |
dc.date.available | 2020-02-27T04:42:45Z | - |
dc.date.created | 2020-02-05 | - |
dc.date.issued | 2019-01-01 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1993 | - |
dc.description.abstract | Natural sources are very promising materials for the discovery of novel bioactive compounds with diverse pharmacological effects. In recent years, many researchers have focused on natural sources as a means to prevent neuronal cell death in neuropathological conditions. This study focused on identifying neuroprotective compounds and their underlying molecular mechanisms. Procyanidin C1 (PC-1) was isolated from grape seeds and assessed for biological effects against glutamate-induced HT22 cell death. The results showed that PC-1 strongly prevented glutamate-induced HT22 cell death. Moreover, PC-1 was also found to prevent glutamate-induced chromatin condensation and reduce the number of annexin V-positive cells indicating apoptotic cell death. Procyanidin C1 possessed a strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and inhibited glutamate-induced accumulation of intracellular reactive oxygen species and protein carbonylation. Additionally, PC-1 mediated nuclear translocation of nuclear factor erythroid-derived 2-related factor 2 and increased the expression levels of heme oxygenase (HO-1). Inhibition of HO-1 by tin protoporphyrin, a synthetic inhibitor, reduced the protective effect of PC-1. Furthermore, PC-1 also blocked glutamate-induced phosphorylation of mitogen-activated protein kinases (MAPKs) including ERK1/2 and p38, but not JNK. This study is the first experimental report to demonstrate the neuroprotective effects of PC-1 against glutamate-induced cytotoxicity in HT22 cells. Therefore, our results suggest that PC-1, as a potent bioactive compound of grape seeds, can prevent neuronal cell death in neuropathological conditions. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | PROTEIN CARBONYLATION | - |
dc.subject | HEME OXYGENASE-1 | - |
dc.subject | BRAIN | - |
dc.subject | NEURODEGENERATION | - |
dc.subject | INHIBITION | - |
dc.subject | MECHANISMS | - |
dc.subject | TOXICITY | - |
dc.subject | NEURONS | - |
dc.subject | KINASE | - |
dc.title | Procyanidin C1 Activates the Nrf2/HO-1 Signaling Pathway to Prevent Glutamate-Induced Apoptotic HT22 Cell Death | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000459747700142 | - |
dc.identifier.doi | 10.3390/ijms20010142 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.20, no.1 | - |
dc.identifier.scopusid | 2-s2.0-85059501896 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 20 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Song, Ji Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Hae-Jeung | - |
dc.contributor.affiliatedAuthor | Kang, Ki Sung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | procyanidin C1 | - |
dc.subject.keywordAuthor | glutamate | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | Nrf2 | - |
dc.subject.keywordAuthor | HO-1 signaling pathway | - |
dc.subject.keywordAuthor | MAPK | - |
dc.subject.keywordAuthor | neuroprotective effects | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | PROTEIN CARBONYLATION | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | NEURODEGENERATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | KINASE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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