Detection and genomic analysis of genital group B streptococcus in pregnant Korean women
- Authors
- Lee, Hwan Tae; Kim, Suk Young; Park, Pil-Whan; Ahn, Jeong-Yeal; Kim, Kyung-Hee; Seo, Ja Young; Jeong, Ji-Hun; Kwoun, Woo-Jae; Seo, Yiel-Hea
- Issue Date
- Jan-2019
- Publisher
- WILEY
- Keywords
- group B streptococcus; molecular serotyping; multilocus sequence typing; real-time polymerase chain reaction
- Citation
- JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, v.45, no.1, pp.69 - 77
- Journal Title
- JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
- Volume
- 45
- Number
- 1
- Start Page
- 69
- End Page
- 77
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/2010
- DOI
- 10.1111/jog.13810
- ISSN
- 1341-8076
- Abstract
- Aim Group B streptococcus (GBS) is a leading cause of life-threatening bacterial infections among newborns, and neonates born to heavily colonized women may be subject to vertical transmission. We sought to determine an appropriate detection method for genital GBS in pregnant women by comparing culture-based methods and real-time polymerase chain reaction (PCR). In addition, we performed molecular serotyping and multilocus sequence typing (MLST) on isolates. Methods A total of 150 pregnant women were enrolled and underwent vaginal-rectal swabbing at 16-40 weeks of gestation. GBS was identified by conventional culture and real-time PCR with or without enrichment. Molecular serotyping and MLST were performed on isolates. Results Overall genital GBS positive rate among the 150 study subjects was 17.3%. Direct culture identified 18 (12.0%) positive specimens, enrichment culture 22 (14.6%), direct PCR 24 (16.0%) and enrichment PCR 25 (16.6%). The sensitivity and specificity by direct and enrichment PCR were as follows: for direct PCR, 90.9% and 96.9%, respectively; and for enrichment PCR, 95.5% and 96.9%, respectively. Resistance rates to clindamycin and erythromycin were 33.3% and 19.1%, respectively. Serotype III-1 was the most common (26.3%), followed by serotype Ib (21.1%), III-3 (15.8%), V (15.8%), II (10.5%), IV (5.3%) and VI (5.3%). Most common sequence types (ST) were ST-1, ST-19 and ST-862 (15.8%), followed by ST-2 and ST-654 (10.5%). Conclusion Direct real-time PCR using vaginal-rectal specimen could be used for detecting GBS in emergent conditions. Molecular serotypes III, Ib and V were most common. MLST analysis frequently presented ST-1, ST-19 and ST-862.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/2010)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.