Atopic Dermatitis-Related Inflammation in Macrophages and Keratinocytes: The Inhibitory Effects of Bee Venom
DC Field | Value | Language |
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dc.contributor.author | 김덕현 | - |
dc.contributor.author | 송호섭 | - |
dc.date.available | 2020-02-27T06:41:38Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 2586-288X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/2499 | - |
dc.description.abstract | Background: This study investigated the anti-inflammatory effects of bee venom (BV) through the inhibition of nuclear factor kappa beta (NF-κB) expression in macrophages and keratinocytes. Methods: Cell viability assays were performed to investigate the cytotoxicity of BV in activated macrophages [lipopolysaccharide (LPS)] and keratinocytes [interferon-gamma/tumor necrosis factor-alpha (IFN-γ/ TNF-α)]. A luciferase assay was performed to investigate the cellular expression of NF-κB in relation to BV dose. The expression of NF-κB inhibitors (p-IκBα, IκBα, and p50 and p65) were determined by Western Blot analysis, and the electromobility shift assay. A nitrite quantification assay was performed to investigate the effect of BV, and NF-κB inhibitor on nitric oxide (NO) production in macrophages. In addition, Western Blot analysis was performed to investigate the effect of BV on the expression of mitogen-activated protein kinases (MAPK) in activated macrophages and keratinocytes. Results: BV was not cytotoxic to activated macrophages and keratinocytes. Transcriptional activity of NFκB, and p50, p65, and p-IκBα expression was reduced by treatment with BV in activated macrophages and keratinocytes. Treatment with BV and an NF-κB inhibitor, reduced the production of NO by activated macrophages, and also reduced NF-κB transcriptional activity in activated keratinocytes (compared with either BV, or NF-κB inhibitor treatment). Furthermore, BV decreased p38, p-p38, JNK, and p-JNK expression in LPS-activated macrophages and IFN-γ/TNF-α-activated keratinocytes. Conclusion: BV blocked the signaling pathway of NF-κB, which plays an important role in the inflammatory response in macrophages and keratinocytes. These findings provided the possibility of BV in the treatment of atopic dermatitis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 대한침구의학회 | - |
dc.relation.isPartOf | Journal of Acupuncture Research | - |
dc.title | Atopic Dermatitis-Related Inflammation in Macrophages and Keratinocytes: The Inhibitory Effects of Bee Venom | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.doi | 10.13045/jar.2019.00038 | - |
dc.identifier.bibliographicCitation | Journal of Acupuncture Research, v.36, no.2, pp.80 - 87 | - |
dc.identifier.kciid | ART002467406 | - |
dc.citation.endPage | 87 | - |
dc.citation.startPage | 80 | - |
dc.citation.title | Journal of Acupuncture Research | - |
dc.citation.volume | 36 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | 김덕현 | - |
dc.contributor.affiliatedAuthor | 송호섭 | - |
dc.subject.keywordAuthor | atopic dermatitis | - |
dc.subject.keywordAuthor | bee venom | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | transcription factor | - |
dc.description.journalRegisteredClass | kci | - |
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