Ginsenoside Rb2 suppresses the glutamate-mediated oxidative stress and neuronal cell death in HT22 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김동휘 | - |
dc.contributor.author | 김대원 | - |
dc.contributor.author | 정보현 | - |
dc.contributor.author | 이종헌 | - |
dc.contributor.author | 이희수 | - |
dc.contributor.author | 황귀서 | - |
dc.contributor.author | 강기성 | - |
dc.contributor.author | 이재욱 | - |
dc.date.available | 2020-02-27T06:41:59Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2019-04 | - |
dc.identifier.issn | 1226-8453 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/2547 | - |
dc.description.abstract | Background: The objective of our study was to analyze the neuroprotective effects of ginsenoside derivatives Rb1, Rb2, Rc, Rd, Rg1, and Rg3 against glutamate-mediated neurotoxicity in HT22 hippocampal mouse neuron cells. Methods: The neuroprotective effect of ginsenosides were evaluated by measuring cell viability. Protein expressions of mitogen-activated protein kinase (MAPK), Bcl2, Bax, and apoptosis-inducing factor (AIF) were determined by Western blot analysis. The occurrence of apoptotic and death cells was determined by flow cytometry. Cellular level of Ca2þ and reactive oxygen species (ROS) levels were evaluated by image analysis using the fluorescent probes Fluor-3 and 20,70-dichlorodihydrofluorescein diacetate, respectively. In vivo efficacy of neuroprotection was evaluated using the Mongolian gerbil of ischemic brain injury model. Result: Reduction of cell viability by glutamate (5 mM) was significantly suppressed by treatment with ginsenoside Rb2. Phosphorylation of MAPKs, Bax, and nuclear AIF was gradually increased by treatment with 5 mM of glutamate and decreased by co-treatment with Rb2. The occurrence of apoptotic cells was decreased by treatment with Rb2 (25.7 mM). Cellular Ca2þ and ROS levels were decreased in the presence of Rb2, and in vivo data indicated that Rb2 treatment (10 mg/kg) significantly diminished the number of degenerated neurons. Conclusion: Our results suggest that Rb2 possesses neuroprotective properties that suppress glutamateinduced neurotoxicity. The molecular mechanism of Rb2 is by suppressing the MAPKs activity and AIF translocation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 고려인삼학회 | - |
dc.relation.isPartOf | Journal of Ginseng Research | - |
dc.title | Ginsenoside Rb2 suppresses the glutamate-mediated oxidative stress and neuronal cell death in HT22 cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000462497500020 | - |
dc.identifier.bibliographicCitation | Journal of Ginseng Research, v.43, no.2, pp.326 - 334 | - |
dc.identifier.kciid | ART002458161 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 334 | - |
dc.citation.startPage | 326 | - |
dc.citation.title | Journal of Ginseng Research | - |
dc.citation.volume | 43 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | 황귀서 | - |
dc.contributor.affiliatedAuthor | 강기성 | - |
dc.subject.keywordAuthor | Ginsenoside Rb2 Neurotoxicity MAPK Reactive oxygen species | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.