Human cytomegalovirus IE86 protein aa 136-289 mediates STING degradation and blocks the cGAS-STING pathway
DC Field | Value | Language |
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dc.contributor.author | Lee, Jun-Kyu | - |
dc.contributor.author | Kim, Jung-Eun | - |
dc.contributor.author | Park, Bang Ju | - |
dc.contributor.author | Song, Yoon-Jae | - |
dc.date.available | 2020-04-06T06:40:25Z | - |
dc.date.created | 2020-04-02 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26185 | - |
dc.description.abstract | We previously reported that human cytomegalovirus (HCMV) 86 kDa immediate-early 2 gene product (IE86) promotes proteasome-dependent degradation of STING. In the present study, we determined the specific residues of IE86 responsible for STING degradation using a STING-firefly luciferase fusion protein expression system for quantitative meas-urement of STING protein levels. IE86 amino acids (aa) 136-289 were sufficient to promote STING degradation and further induced down-regulation of 2 ' 3 '-cyclic GMP-AMP (cGAMP)-mediated IFN-beta promoter activation. Interestingly, transactivation domains (TAD) of the IE86 protein located at the N- and C-termini were required for down-regulation of Toll/interleukin-1 receptor (TIR) domain-containing adaptor-inducing interferon beta (IFN-beta) (TRIF)-mediated IFN-beta-and p65/RelA-induced NF-kappa B-dependent promoter activation while amino acids (aa) 136-289 had no significant effects. Our collective data suggest that the IE86 protein utilizes the aa 136-289 region to promote STING degradation and inhibit the cGAS-STING pathway. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY | - |
dc.subject | CYCLIC DINUCLEOTIDE | - |
dc.subject | VIRUS | - |
dc.subject | REQUIRES | - |
dc.title | Human cytomegalovirus IE86 protein aa 136-289 mediates STING degradation and blocks the cGAS-STING pathway | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000511694400008 | - |
dc.identifier.doi | 10.1007/s12275-020-9577-6 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.58, no.1, pp.54 - 60 | - |
dc.identifier.kciid | ART002537605 | - |
dc.identifier.scopusid | 2-s2.0-85077188614 | - |
dc.citation.endPage | 60 | - |
dc.citation.startPage | 54 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 58 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Lee, Jun-Kyu | - |
dc.contributor.affiliatedAuthor | Kim, Jung-Eun | - |
dc.contributor.affiliatedAuthor | Park, Bang Ju | - |
dc.contributor.affiliatedAuthor | Song, Yoon-Jae | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | HCMV | - |
dc.subject.keywordAuthor | IE86 | - |
dc.subject.keywordAuthor | STING | - |
dc.subject.keywordAuthor | type I IFN | - |
dc.subject.keywordPlus | CYCLIC DINUCLEOTIDE | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | REQUIRES | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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