A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles
DC Field | Value | Language |
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dc.contributor.author | Thao Nguyen Le | - |
dc.contributor.author | Tai Duc Tran | - |
dc.contributor.author | Moon Il Kim | - |
dc.date.available | 2020-04-06T06:40:31Z | - |
dc.date.created | 2020-04-02 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 2079-4991 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26188 | - |
dc.description.abstract | An effective novel strategy to detect bacteria is promising because it may improve human health by allowing early diagnosis and timely treatment of bacterial infections. Here, we report a simple, reliable, and economical colorimetric assay using the peroxidase-like activity of chitosan-coated iron oxide magnetic nanoparticles (CS-MNPs). When CS-MNPs are incubated with a sample containing bacterial cells such as the gram-negative Escherichia coli or the gram-positive Staphylococcus aureus, the negatively-charged bacterial membrane interacts with positively-charged chitosan on the surface of CS-MNPs, thus resulting in significant reduction of their peroxidase-like activity presumably by a hindrance in the accessibility of the negatively charged substrate, 2-2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) to the positively-charged CS-MNPs. This simple colorimetric strategy allowed the rapid detection of bacterial cells down to 10(4) CFU mL(-1) by the naked eye and 10(2) CFU mL(-1) by spectrophotometry within 10 min. Based on the results, we anticipate that the CS-MNPs-based assay has great potential for the on-site diagnosis of bacterial infections in facility-limited or point-of-care testing (POCT) environments. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | NANOMATERIALS | - |
dc.title | A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000516825600092 | - |
dc.identifier.doi | 10.3390/nano10010092 | - |
dc.identifier.bibliographicCitation | NANOMATERIALS, v.10, no.1 | - |
dc.identifier.scopusid | 2-s2.0-85078259746 | - |
dc.citation.title | NANOMATERIALS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Thao Nguyen Le | - |
dc.contributor.affiliatedAuthor | Tai Duc Tran | - |
dc.contributor.affiliatedAuthor | Moon Il Kim | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | colorimetric bacteria detection | - |
dc.subject.keywordAuthor | chitosan-coated magnetic nanoparticles | - |
dc.subject.keywordAuthor | peroxidase-like activity | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | Staphylococcus aureus | - |
dc.subject.keywordPlus | FE3O4 NANOPARTICLES | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | REMOVAL | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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