Unique triterpenoid of jujube root protects cisplatin-induced damage in kidney epithelial LLC-PK1 cells via autophagy regulation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee D. | - |
dc.contributor.author | Kang K.B. | - |
dc.contributor.author | Kim H.W. | - |
dc.contributor.author | Park J.S. | - |
dc.contributor.author | Hwang G.S. | - |
dc.contributor.author | Kang K.S. | - |
dc.contributor.author | Choi S. | - |
dc.contributor.author | Yamabe N. | - |
dc.contributor.author | Kim K.H. | - |
dc.date.available | 2020-04-06T07:37:03Z | - |
dc.date.created | 2020-04-02 | - |
dc.date.issued | 2020-03 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26387 | - |
dc.description.abstract | Chronic exposure to cisplatin is associated with irreversible kidney impairment. In this present study, we explored the protective effects of 3-dehydroxyceanothetric acid 2-methyl ester (3DC2ME) isolated from roots of jujube (Ziziphus jujuba, Rhamnaceae) against cisplatin-induced damage in vitro. In kidney epithelial LLC-PK1 cells, western blotting and staining with specific autophagy epifluorescent dye CytoID were used to determine the molecular pathways involving autophagy. Treatment with 3DC2ME reduced the increased Cyto-ID-stained autophagic vesicles and reversed the protein expressions of 5’ AMP-activated protein kinase subunit β-1 (AMPK)/mammalian target of rapamycin (mTOR)-dependent signaling pathway in cisplatin-induced cell death. Additionally, treatment with autophagy inhibitor 3-methyladenine (3-MA) and with or without 3DC2ME attenuated the cisplatin-induced apoptosis. Although further research is necessary to substantiate the effects, we evaluated the potential mechanism of action of 3DC2ME as an adjuvant for cancer patients. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI AG | - |
dc.relation.isPartOf | Nutrients | - |
dc.title | Unique triterpenoid of jujube root protects cisplatin-induced damage in kidney epithelial LLC-PK1 cells via autophagy regulation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000531831000086 | - |
dc.identifier.doi | 10.3390/nu12030677 | - |
dc.identifier.bibliographicCitation | Nutrients, v.12, no.3 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85081041270 | - |
dc.citation.title | Nutrients | - |
dc.citation.volume | 12 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Park J.S. | - |
dc.contributor.affiliatedAuthor | Hwang G.S. | - |
dc.contributor.affiliatedAuthor | Kang K.S. | - |
dc.contributor.affiliatedAuthor | Choi S. | - |
dc.contributor.affiliatedAuthor | Yamabe N. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Autophagy | - |
dc.subject.keywordAuthor | Cisplatin | - |
dc.subject.keywordAuthor | Nephrotoxicity | - |
dc.subject.keywordAuthor | Ziziphus jujuba | - |
dc.subject.keywordPlus | 3 dehydroxyceanothetric acid 2 methyl ester | - |
dc.subject.keywordPlus | 3 methyladenine | - |
dc.subject.keywordPlus | acetylcysteine | - |
dc.subject.keywordPlus | cisplatin | - |
dc.subject.keywordPlus | jujube extract | - |
dc.subject.keywordPlus | triterpenoid | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | AMPK signaling | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | autophagosome | - |
dc.subject.keywordPlus | autophagy (cellular) | - |
dc.subject.keywordPlus | cell viability | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | cytotoxicity assay | - |
dc.subject.keywordPlus | fluorescence microscopy | - |
dc.subject.keywordPlus | image cytometry | - |
dc.subject.keywordPlus | jujube | - |
dc.subject.keywordPlus | kidney injury | - |
dc.subject.keywordPlus | liquid chromatography-mass spectrometry | - |
dc.subject.keywordPlus | LLC-PK1 cell line | - |
dc.subject.keywordPlus | metabolic regulation | - |
dc.subject.keywordPlus | mTOR signaling | - |
dc.subject.keywordPlus | nephrotoxicity | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | nuclear magnetic resonance spectroscopy | - |
dc.subject.keywordPlus | plant root | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | radioimmunoprecipitation | - |
dc.subject.keywordPlus | renal protection | - |
dc.subject.keywordPlus | Western blotting | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.