Effect of exposure to Asian sand dust-Particulate matter on liver Tenascin-C expression in human cancer cell and mouse hepatic tissue
DC Field | Value | Language |
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dc.contributor.author | Lee, Yong Hyun | - |
dc.contributor.author | Kim, Dae Young | - |
dc.contributor.author | Jeong, Sung Hwan | - |
dc.contributor.author | Hwang, You Jin | - |
dc.date.available | 2020-02-27T07:41:23Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2019-09 | - |
dc.identifier.issn | 1880-3989 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/2710 | - |
dc.description.abstract | Asian Sand Dust-Particulate Matter (ASD-PM) aerosol brings large amounts of wind-eroded soil particles containing high concentrations of metallic components caused by industrialization and vehicles. Proinflammatory and cytotoxic cytokines trigger local inflammatory responses and cause a systematically high incidence of cardiovascular and other diseases. Tenascin C (Tn-C) is known to be expressed in damaged tissue or in a developmental stage of tissue. In this study, we examined the expression of Tn-C and Fibronectin in human cancer-cell lines and in liver tissue of mice treated with ASD-PM to investigate the inflammatory and cell-damage effects of ASD-PM. In our in vivo study, mice were intratracheally instilled with saline suspensions of ASD-PM particles. Instillation of these particles was repeated twice a week for 12 weeks and the liver tissues were stained with hematoxylin, eosin, and Masson's trichrome, and we carried out an IF. Tn-C expression in liver tissues was detected by RT-PCR and western blot analysis. In the results, the expression of Tn-C increased in a dose-dependent manner in both RNA and Immunofluorescence assay (IF). In our in vitro study, A549 and Hep3B cell lines were incubated in culture media with Transforming Growth Factor-Beta1(TGF-β1) and ASD-PM. Immunofluorescence microscopy images showed a two times stronger expression of fluorescence in the ASD-treated group than in that treated with TGF-β1. They also showed a stronger expression of Tn-C in proportion to the concentration of ASD-PM. We confirmed that ASD-PM when inhaled formally migrated to other organs and induced Tn-C expression. ASD-PM containing metals causes expression of Tn-C in liver tissue in proportion to the concentration of ASD-PM. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | JAPANESE SOC TOXICOLOGICAL SCIENCES | - |
dc.relation.isPartOf | The Journal of toxicological sciences | - |
dc.title | Effect of exposure to Asian sand dust-Particulate matter on liver Tenascin-C expression in human cancer cell and mouse hepatic tissue | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000514252200019 | - |
dc.identifier.doi | 10.2131/jts.44.633 | - |
dc.identifier.bibliographicCitation | The Journal of toxicological sciences, v.44, no.9, pp.633 - 641 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85071767691 | - |
dc.citation.endPage | 641 | - |
dc.citation.startPage | 633 | - |
dc.citation.title | The Journal of toxicological sciences | - |
dc.citation.volume | 44 | - |
dc.citation.number | 9 | - |
dc.contributor.affiliatedAuthor | Lee, Yong Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Dae Young | - |
dc.contributor.affiliatedAuthor | Jeong, Sung Hwan | - |
dc.contributor.affiliatedAuthor | Hwang, You Jin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Asian sand dust (ASD) - Particulate matter (PM) | - |
dc.subject.keywordAuthor | Fibrosis | - |
dc.subject.keywordAuthor | Tenascin-C (Tn-C) | - |
dc.subject.keywordAuthor | TGF-β1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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