Glucagon-Like Peptide 1 Increases beta-Cell Regeneration by Promoting alpha- to beta-Cell Transdifferentiation
DC Field | Value | Language |
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dc.contributor.author | Lee, Young-Sun | - |
dc.contributor.author | Lee, Changmi | - |
dc.contributor.author | Choung, Jin-Seung | - |
dc.contributor.author | Jung, Hye-Seung | - |
dc.contributor.author | Jun, Hee-Sook | - |
dc.date.available | 2020-02-27T08:41:08Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-12-01 | - |
dc.identifier.issn | 0012-1797 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/2982 | - |
dc.description.abstract | Glucagon-like peptide 1 (GLP-1) can increase pancreatic -cells, and -cells could be a source for new -cell generation. We investigated whether GLP-1 increases -cells through -cell transdifferentiation. New -cells originating from non--cells were significantly increased in recombinant adenovirus expressing GLP-1 (rAd-GLP-1)-treated RIP-CreER;R26-YFP mice. Proliferating -cells were increased in islets of rAd-GLP-1-treated mice and TC1 clone 9 (TC1-9) cells treated with exendin-4, a GLP-1 receptor agonist. Insulin(+)glucagon(+) cells were significantly increased by rAd-GLP-1 or exendin-4 treatment in vivo and in vitro. Lineage tracing to label the glucagon-producing -cells showed a higher proportion of regenerated -cells from -cells in rAd-GLP-1-treated Glucagon-rtTA;Tet-O-Cre;R26-YFP mice than rAd producing -galactosidase-treated mice. In addition, exendin-4 increased the expression and secretion of fibroblast growth factor 21 (FGF21) in TC1-9 cells and -cell-ablated islets. FGF21 treatment of -cell-ablated islets increased the expression of pancreatic and duodenal homeobox-1 and neurogenin-3 and significantly increased insulin(+)glucagon(+) cells. Generation of insulin(+)glucagon(+) cells by exendin-4 was significantly reduced in islets transfected with FGF21 small interfering RNA or islets of FGF21 knockout mice. Generation of insulin(+) cells by rAd-GLP-1 treatment was significantly reduced in FGF21 knockout mice compared with wild-type mice. We suggest that GLP-1 has an important role in -cell transdifferentiation to generate new -cells, which might be mediated, in part, by FGF21 induction. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER DIABETES ASSOC | - |
dc.relation.isPartOf | DIABETES | - |
dc.subject | INSULIN-PRODUCING CELLS | - |
dc.subject | DIABETIC MICE | - |
dc.subject | TRANSCRIPTION FACTORS | - |
dc.subject | ANTIDIABETIC ACTIONS | - |
dc.subject | IN-VITRO | - |
dc.subject | EXPRESSION | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | ACTIVATION | - |
dc.subject | PDX1 | - |
dc.subject | SENSITIVITY | - |
dc.title | Glucagon-Like Peptide 1 Increases beta-Cell Regeneration by Promoting alpha- to beta-Cell Transdifferentiation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000450561900012 | - |
dc.identifier.doi | 10.2337/db18-0155 | - |
dc.identifier.bibliographicCitation | DIABETES, v.67, no.12, pp.2601 - 2614 | - |
dc.identifier.scopusid | 2-s2.0-85056803173 | - |
dc.citation.endPage | 2614 | - |
dc.citation.startPage | 2601 | - |
dc.citation.title | DIABETES | - |
dc.citation.volume | 67 | - |
dc.citation.number | 12 | - |
dc.contributor.affiliatedAuthor | Lee, Young-Sun | - |
dc.contributor.affiliatedAuthor | Lee, Changmi | - |
dc.contributor.affiliatedAuthor | Choung, Jin-Seung | - |
dc.contributor.affiliatedAuthor | Jun, Hee-Sook | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INSULIN-PRODUCING CELLS | - |
dc.subject.keywordPlus | DIABETIC MICE | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTORS | - |
dc.subject.keywordPlus | ANTIDIABETIC ACTIONS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PDX1 | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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